System for and method of processing soft tissue and skin with fluids using temperature and pressure changes
A method of processing soft tissue and skin using supercritical fluids is disclosed. The method comprises placing the soft tissue or skin on a scaffold in a processing chamber, adding supercritical fluid to the processing chamber, and pulsing the supercritical fluid in the processing chamber. A processing system for processing soft tissue and skin using supercritical fluids in accordance with the present invention comprises a processing chamber for housing the soft tissue, a scaffold, a vat for storing a processing fluid, a pump, a heating element, and a flow path.
1. A method of processing soft tissue comprising:
a) providing soft tissue, wherein the soft tissue contains contaminants;
b) coupling the soft tissue to a scaffold positioned within a processing chamber;
c) introducing only supercritical CO 2 and a degreasing agent into the processing chamber, wherein the degreasing agent is an alcohol or acetone; and
d) performing a pulsing step on the supercritical CO 2 and degreasing agent, wherein the pulsing step comprises fluctuating pressure of the supercritical CO 2 and degreasing agent, wherein the supercritical CO 2 and degreasing agent fluctuates between its supercritical and nonsupercritical states, and wherein the pulsing step kills substantially all the contaminants present within the soft tissue.
2. The method of claim 1 , wherein the pulsing step agitates the contaminants present in the soft tissue, thereby removing the contaminants from the soft tissue.
3. The method of claim 1 , wherein the degreasing agent is acetone.
4. The method of claim 1 , further comprising:
introducing a drying chemistry into the processing chamber after the pulsing step, wherein the drying chemistry comprises a supercritical fluid and a drying agent.
5. The method of claim 4 , wherein the drying agent is acetone.
6. The method of claim 1 , further comprising:
exposing the soft tissue to gamma-ray radiation.
7. The method of claim 1 , further comprising:
a) removing the fluid from the processing chamber into a recirculation loop;
b) filtering the fluid in the recirculation loop, forming a filtered fluid; and
c) re-introducing the filtered fluid into the processing chamber.
8. The method of claim 1 , further comprising:
a) introducing at least one additional portion of fluid into the processing chamber;
b) performing at least one additional pulsing step on the at least one additional portion of fluid.
9. A method of processing soft tissue comprising:
a) providing soft tissue, wherein the soft tissue contains contaminants;
b) coupling the soft tissue to a scaffold positioned within a processing chamber;
c) introducing cryogenic CO 2 into the processing chamber;
d) performing a pulsing step on the cryogenic CO 2 , wherein the pulsing step comprises heating the cryogenic CO 2 to a supercritical state, forming supercritical CO 2 , wherein the supercritical CO 2 fluctuates between its cryogenic and supercritical states, and wherein the pulsing step kills substantially all the contaminants present within the soft tissue.
10. A method of processing soft tissue comprising:
a) providing soft tissue, wherein the soft tissue contains contaminants;
b) coupling the soft tissue to a scaffold positioned within a processing chamber;
c) introducing supercritical CO 2 into the processing chamber; and
d) performing a pulsing step on the supercritical CO 2 , wherein the pulsing step comprises fluctuating pressure of the supercritical CO 2 , wherein the supercritical CO2 fluctuates between its supercritical and nonsupercritical states, and wherein the pulsing step kills substantially all the contaminants present within the soft tissue.
11. The method of claim 9 , further comprising:
introducing a drying chemistry into the processing chamber after the pulsing step, wherein the drying chemistry comprises a supercritical fluid and a drying agent.
12. The method of claim 11 , wherein the drying agent is acetone.
13. The method of claim 9 , further comprising:
exposing the soft tissue to gamma-ray radiation.
14. The method of claim 10 , further comprising:
introducing a drying chemistry into the processing chamber after the pulsing step, wherein the drying chemistry comprises a supercritical fluid and a drying agent.
15. The method of claim 14 , wherein the drying agent is acetone.
16. The method of claim 10 , further comprising:
exposing the soft tissue to gamma-ray radiation.