Fluorescent ion indicators for cadmium and lanthanide ion detection
View Patent ↗The present invention provides a metal chelator and methods that facilitate binding, detecting, monitoring and quantitating of heavy metal ions in a sample. This metal chelating moiety has the following formula
1. A compound having the formula:
2. A complex comprising a metal ion and the compound according to claim 1 .
3. The complex according to claim 2 , wherein the metal ion is lead, cadmium, nickel, lanthanum or mercury.
4. A composition of matter comprising a sample and the compound according to claim 1 .
5. The composition according to claim 4 , wherein the sample comprises living cells, cellular components, proteins, peptides, buffer solutions, intracellular fluids, extracellular fluids, fixed cells, biological fermentation media, environmental sample, industrial samples or chemical reactors, eukaryotic cells, or prokaryotic cells.
6. The composition according to claim 4 , wherein the sample comprises blood cells, immune cells, cultured cells, muscle tissue, neurons, extracellular vesicles; vascular tissue, blood fluids, saliva, urine; water, soil, waste water, sea water; pharmaceuticals, foodstuffs or beverages.
7. A method for detecting the presence or absence of a metal ion is a sample, wherein the method comprises:
a. combining the compound according to claim 1 with the sample to prepare a labeling mixture,
b. incubating the labeling mixture for a sufficient amount of time for the compound to associate with the metal ion in the sample to form an incubated mixture;
c. illuminating the incubated sample with an appropriate wavelength to form an illuminated mixture; and,
d. observing the illuminated mixture whereby the presence or absence of the metal ion in a sample is detected.
8. The method according to claim 7 , wherein the metal ion is lead, cadmium, nickel, lanthanum or mercury.
9. The method according to claim 7 , wherein the sample comprises living cells, cellular components, proteins, peptides, buffer solutions, intracellular fluids, extracellular fluids, fixed cells, biological fermentation media, environmental sample, industrial samples or chemical reactors, eukaryotic cells, or prokaryotic cells.
10. The method according to claim 7 , wherein the sample comprises blood cells, immune cells, cultured cells, muscle tissue, neurons, extracellular vesicles; vascular tissue, blood fluids, saliva, urine; water, soil, waste water, sea water; pharmaceuticals, foodstuffs or beverages.