Generation of plants with improved drought tolerance
View Patent ↗The present invention is directed to plants that display a pathogen resistance and increased drought tolerance phenotype due to altered expression of a PRDT1 nucleic acid. The invention is further directed to methods of generating plants with a pathogen resistance and increased drought tolerance phenotype.
1. A method of producing a plant having increased drought tolerance, said method comprising:
a) introducing into cells of the plant a plant transformation vector comprising a nucleotide sequence that encodes a myb polypeptide that confers drought tolerance, said myb polypeptide selected from the group consisting of: the polypeptide sequence of SEQ ID NO:2, a polypeptide sequence having at least 95% sequence identity to SEQ ID NO: 2, and an Arabidopsis thaliana polypeptide having at least 85% sequence identity to SEQ ID NO: 2; and
b) growing the transformed cells to produce a transgenic plant, wherein said nucleotide sequence is expressed, and said transgenic plant exhibits increased drought tolerance, relative to a control plant.
2. A transformed plant obtained by the method of claim 1 .
3. A transformed plant part obtained from the plant according to claim 2 .
4. The transformed plant of claim 2 , wherein the nucleotide sequence is set forth in SEQ ID NO:1.
5. The transformed plant of claim 2 , wherein the nucleotide sequence encodes an Arabidopsis thaliana myb polypeptide having at least 85% sequence identity to SEQ ID NO: 2.
6. The transformed plant of claim 5 , wherein the Arabidopsis thaliana myb polypeptide has at least 90% sequence identity to SEQ ID NO: 2.
7. A method of producing a plant having increased drought tolerance, said method comprising:
a) introducing into cells of the plant a plant transformation vector comprising a nucleotide sequence that encodes a drought tolerance polypeptide comprising the amino acid sequence of SEQ ID NO: 2 or SEQ ID NO: 13; and
b) growing the transformed cells to produce a transgenic plant, wherein said nucleotide sequence is expressed, and said transgenic plant exhibits increased drought tolerance relative to a control plant.
8. A transformed plant produced by the method of claim 7 .
9. The transformed plant of claim 8 , wherein the nucleotide sequence encodes SEQ ID NO: 2.
10. The transformed plant of claim 9 , wherein the nucleotide sequence comprises SEQ ID NO: 1.
11. The method of claim 1 , wherein the transformation vector further comprises a constitutive promoter operably linked to the nucleotide sequence.
12. The method of claim 1 , wherein the transformation vector further comprises a drought inducible promoter operably linked to the nucleotide sequence.
13. The transformed plant of claim 6 , wherein the nucleotide sequence encodes an Arabidopsis thaliana myb polypeptide having at least 95% sequence identity to SEQ ID NO: 2.