Docetaxel Immunoassay
Novel conjugates of docetaxel and novel docetaxel immunogens derived from the 7 and 10 positions of docetaxel and monoclonal antibodies generated by these docetaxel linked immunogens are useful in immunoassays for the quantification and monitoring of docetaxel in biological fluids.
1 - 17 . (canceled)
18 . An antibody which reacts with docetaxel and does not substantially react with taxol.
19 . The antibody of claim 18 , wherein said antibody is generated from an immunogen comprising an immunogenic carrier linked to a ligand selected from the group consisting of a compound of the formula:
wherein
Y is an organic spacing group;
X is a functional group capable of binding to a carrier; and
p is an integer from 0 to 1;
a compound of the formula:
wherein B, X, Y and p are as above;
a compound of the formula
wherein B, X, Y and p are as above;
and mixtures thereof.
20 . The antibody of claim 19 , wherein said antibody is derived from mice, rabbits or rats.
21 . The antibody of claim 20 , wherein said antibody is a monoclonal antibody.
22 . The antibody of claim 19 , wherein said antibody is derived from an immunogen of an immunogenic carrier with a ligand of the formula:
wherein X, Y, B and p are as above.
23 . The antibody of claim 22 , wherein said antibody is derived from mice, rabbits or rats.
24 . The antibody of claim 23 , wherein said antibody is a monoclonal antibody.
25 . The antibody of claim 19 , wherein said antibody is derived from an immunogen of a polyamine polymer with a ligand of the formula:
wherein B, X, Y and p are as above.
26 . The antibody of claim 25 , wherein said antibody is derived from mice, rabbits or rats.
27 . The antibody of claim 25 , wherein said antibody is a monoclonal antibody.
28 . The antibody of claim 27 , wherein said antibody is derived from mice, rabbits or rats.
29 . The antibody of claim 19 , wherein said antibody is derived from an immunogen of a carrier with a ligand of the formula:
wherein B, X, Y and p are as above.
30 . The antibody of claim 29 , wherein said antibody is derived from mice, rabbits or rats.
31 . The antibody of claim 30 , wherein said antibody is a monoclonal antibody.
32 . The antibody of claim 31 , wherein said antibody is derived from mice, rabbits or rats.
33 . A compound of the formula:
wherein
Y is an organic spacing group;
X is a functional group capable of binding to a carrier; and
p is an integer from 0 to 1.
34 . The compound of claim 33 , wherein said carrier contains a polyamine polymer.
35 . The compound of claim 34 , wherein p is 0.
36 . The compound of claim 35 , wherein X is
wherein R 3 is hydrogen or taken together with its attached oxygen atom forms a reactive ester and R 4 is oxygen or sulfur.
37 . The compound of claim 36 , wherein
and R 3 hydrogen.
38 . The compound of claim 36 , wherein
and R 3 forms a reactive ester.
39 . The compound of claim 38 , wherein the ester formed is a lower alkyl ester, imidoester or amidoester.
40 . The compound of claim 34 , wherein p is 1.
41 . The compound of claim 40 , wherein X is
wherein R 3 is hydrogen or taken together with its attached oxygen atom forms a reactive ester and R 4 is oxygen or sulfur.
42 . The compound of claim 41 , wherein Y is alkylene containing from 1 to 10 carbon atoms,
wherein n and o are integers from 0 to 6, and m is an integer from 1 to 6.
43 . A conjugate comprising a carrier linked to a ligand moiety of the formula:
wherein
Y is an organic spacing group;
X′ is —CH2- or a functional linking group capable of linking to a carrier; and
p is an integer from 0 to 1.
44 . The conjugate of claim 43 , wherein the carrier comprises a polyamine polymer.
45 . The conjugate of claim 44 , wherein p is 1.
46 . The conjugate of claim 45 , wherein
wherein R 4 is oxygen or sulfur.
47 . The conjugate of claim 44 , wherein p is 1.
48 . The conjugate of claim 45 , wherein Y is alkylene containing from 1 to 10 carbon atoms,
wherein n and o are integers from 0 to 6, and m is an integer from 1 to 6.
49 . The conjugate of claim 48 , wherein
wherein R 4 is oxygen or sulfur.
50 . The conjugate of claim 44 , wherein said polyamine polymer is an immunogenic polymer.
51 . A compound of the formula:
wherein
Y is an organic spacing group;
X is a functional group capable of binding to a carrier; and
p is an integer from 0 to 1.
52 . The compound of claim 51 , wherein X is a functional group capable of binding to a polyamine polymer.
53 . The compound of claim 52 , wherein p is 0.
54 . The compound of claim 52 , wherein X is
wherein R 3 is hydrogen or taken together with its attached oxygen atom forms a reactive ester and R 4 is oxygen or sulfur.
55 . The compound of claim 54 , wherein
and R 3 is hydrogen.
56 . The compound of claim 54 , wherein
and R 3 forms a reactive ester.
57 . The compound of claim 56 , wherein the ester formed is a lower alkyl ester, imidoester or amidoester.
58 . The compound of claim 51 , wherein p is 1.
59 . The compound of claim 58 , wherein Y is alkylene containing from 1 to 10 carbon atoms,
wherein n and o are integers from 0 to 6, and m is an integer from 1 to 6.
60 . A compound of the formula:
wherein
Y is an organic spacing group;
X is a functional group capable of binding to a carrier; and
p is an integer from 0 to 1.
61 . The compound of claim 60 , wherein X is capable of binding to a polyamine polymer.
62 . The compound of claim 61 , wherein p is 0.
63 . The compound of claim 62 , wherein X is
wherein R 3 is hydrogen or taken together with its attached oxygen atom forms a reactive ester and R 4 is oxygen or sulfur.
64 . The compound of claim 63 , wherein
and R 3 is hydrogen.
65 . The compound of claim 63 , wherein
and R 3 forms a reactive ester.
66 . The compound of claim 65 , wherein the ester formed is a lower alkyl ester, imidoester or amidoester.
67 . The compound of claim 61 , wherein p is 1.
68 . The compound of claim 67 , wherein X is
wherein R 3 is hydrogen or taken together with its attached oxygen atom forms a reactive ester and R 4 is oxygen or sulfur.
69 . The compound of claim 68 , wherein Y is alkylene containing from 1 to 10 carbon atoms,
wherein n and o are integers from 0 to 6, and m is an integer from 1 to 6.
70 . A conjugate comprising a carrier linked to a ligand moiety of the formula:
wherein
Y is an organic spacing group;
X′ is —CH2- or a functional linking group capable of linking to a carrier; and
p is an integer from 0 to 1.
71 . The conjugate of claim 70 , wherein the carrier comprises a polyamine polymer.
72 . The conjugate of claim 71 , wherein p is 0.
73 . The conjugate of claim 72 , wherein
wherein R 4 is oxygen or sulfur.
74 . The conjugate of claim 71 , wherein p is 1.
75 . The conjugate of claim 74 , wherein Y is alkylene containing from 1 to 10 carbon atoms,
wherein n and o are integers from 0 to 6, and m is an integer from 1 to 6.
76 . The conjugate of claim 75 , wherein
wherein R 4 is oxygen or sulfur.
77 . The conjugate of claim 71 , wherein said polyamine polymer is an immunogenic polymer.
78 . A conjugate comprising a carrier linked to a ligand moiety of the formula:
wherein
Y is an organic spacing group;
X′ is —CH 2 — or a functional linking group capable of linking to said carrier; and
p is an integer from 0 to 1.
79 . The conjugate of claim 78 , wherein the carrier comprises a polyamine polymer.
80 . The compound of claim 79 , wherein p is 0.
81 . The compound of claim 80 , wherein
wherein R 4 is oxygen or sulfur.
82 . The conjugate of claim 79 , wherein p is 1.
83 . The conjugate of claim 82 , wherein Y is alkylene containing from 1 to 10 carbon atoms,
wherein n and o are integers from 0 to 6, and m is an integer from 1 to 6.
84 . The conjugate of claim 83 , wherein
wherein R 4 is oxygen or sulfur.
85 . The conjugate of claim 79 , wherein said polyamine polymer is an immunogenic polymer.
86 . (canceled)