Isolation of skeletal precursor cells
View Patent ↗Cartilage-derived morphogenetic protein CDMP-1 or a transforming growth factor β having at least 80% homology with CDMP-1, or a factor co-expressed and/or co-detectable therewith, is used as a marker of skeletal precursor cells from any part of a mammalian body.
1. A method of identifying viable, expanded or passaged, committed skeletal precursor cells capable of differentiating into skeletal tissues comprising the step of:
detecting the expression of cartilage-derived morphogenic protein-1 (CDMP-1) in an in vitro cell culture of cells obtained from human periosteum, synovial membrane or bone marrow,
wherein the expression of CDMP-1 identifies skeletal precursor cells.
2. The method according to claim 1 , wherein the expression of CDMP-1 is detected at the DNA, mRNA, cDNA or protein level and/or via the activity of a CDMP-1 promoter that is operably linked to a heterologous reporter gene.
3. The method according to claim 1 , wherein the step of detecting CDMP-1 expression includes applying a binding agent for CDMP-1 to the in vitro cell culture.
4. The method according to claim 1 , which further comprises detecting the absence of expression of a marker selected from the group consisting of FGFR3, type II collagen, type IX collagen, type X collagen, type XI collagen, and BMP-2.
5. A method of obtaining a homogenous culture of viable, expanded or passaged, committed skeletal precursor cells capable of differentiating into skeletal tissues comprising the steps of:
a) detecting the expression of cartilage-derived morphogenic protein-1 (CDMP-1) in an in vitro cell culture of cells obtained from human periosteum, synovial membrane or bone marrow; and
b) selecting or enriching CDMP-1 positive cells, thereby producing a homogenous culture of skeletal precursor cells capable of differentiating into skeletal tissue.
6. The method according to claim 5 wherein the expression of CDMP-1 is detected at the DNA, mRNA, cDNA or protein level and/or via the activity of a CDMP-1 promoter that is operably linked to a heterologous reporter gene.
7. The method according to claim 5 , wherein the step of detecting CDMP-1 expression includes applying a binding agent for CDMP-1 to the in vitro cell culture.
8. The method according to claim 5 , which further comprises detecting the absence of expression of a marker selected from the group consisting of FGFR3, type II collagen, type IX collagen, type X collagen, type XI collagen, and BMP-2.