System and Methods for Determination of Analyte Concentration Using Time Resolved Amperometry
This invention is a method for determining a concentration of an analyte. The steps include applying a potential excitation to a fluid sample containing an analyte, and measuring one or more currents associated with one or more time-segments. The method can also include calculating a final analyte concentration based on a first and second set of analyte concentrations, wherein each set of analyte concentration values is based on a first and second set of calibration data associated with first and second time-segments.
1 . A method for analyzing an analyte, the steps comprising:
applying a potential excitation to a fluid sample containing an analyte;
measuring a first current during a first time-segment following application of the potential excitation;
measuring a second current during a second time-segment following application of the potential excitation;
calculating a plurality of first analyte concentrations based on the first measured current and a first set of calibration data associated with the first time-segment; and
calculating a plurality of second analyte concentrations based on the second measured current and a second set of calibration data associated with the second time-segment.
2 . The method of claim 1 , further comprising:
determining a final analyte concentration based on at least one of the first analyte concentrations and at least one of the second analyte concentrations.
3 . The method of claim 2 , wherein determining the final analyte concentration includes at least one of an iterative algorithm, an interpolative algorithm, a line-plot, and an extrapolative algorithm.
4 . The method of claim 1 , wherein a set of calibration data includes at least one of a plurality of calibration curves, a lookup table, a data array, and a mathematical equation.
5 . The method of claim 1 , wherein the first time-segment is less than about two to ten seconds, and the second time-segment is more than about two to ten seconds.
6 . The method of claim 5 , wherein the first time-segment is less than about eight seconds, and the second time-segment is more than about eight seconds.
7 . The method of claim 1 , wherein at least part of the calibration data is determined using at least one of empirical data, and predicted data.
8 . The method of claim 1 , wherein the first and second sets of calibration data are associated with different levels of hematocrit.
9 . The method of claim 8 , wherein the different levels of hematocrit include at least one of a high level greater than about 42%, a physiological level of about 42%, and a low level less than about 42%.
10 . The method of claim 1 , further comprising:
measuring a third current during a third time-segment following application of the potential excitation;
calculating a plurality of third analyte concentrations based on the third measured current and a third set of calibration data associated with the third time-segment; and
determining a final analyte concentration based on at least one of the first and second analyte concentrations and at least one of the third analyte concentrations.
11 . The method of claim 1 , wherein the analyte is glucose and the fluid sample includes blood.
12 . The method of claim 1 , wherein the fluid sample includes an enzyme of at least one of glucose oxidase and glucose dehydrogenase and a mediator of at least one of potassium ferricyanide and ruthenium hexamine.
13 . A system for analyzing an analyte in a fluid sample, comprising:
a set of electrodes configured to apply a potential excitation to a fluid sample containing an analyte;
a processor configured to:
measure a first current during a first time-segment following application of the potential excitation;
measure a second current during a second time-segment following application of the potential excitation;
calculate a plurality of first analyte concentrations based on the first measured current and a plurality of first calibration curves associated with the first time-segment; and
calculate a plurality of second analyte concentrations based on the second measured current and a plurality of second calibration curves associated with the second time-segment.
14 . The system of claim 13 , further comprising a processor configured to:
determine a final analyte concentration based on at least one of the first analyte concentrations and at least one of the second analyte concentrations.
15 . The system of claim 14 , wherein the final analyte concentration is determined using at least one of an iterative algorithm, an interpolative algorithm, a line-plot, and an extrapolative algorithm.
16 . The system of claim 14 , wherein the system is further configured to a display a value representative of the final analyte concentration.
17 . The system of claim 13 , wherein the first time-segment is less than about two to ten seconds, and the second time-segment is more than about two to ten seconds.
18 . The system of claim 13 , wherein the first time-segment is less than about eight seconds, and the second time-segment is more than about eight seconds.
19 . The system of claim 13 , wherein at least one of the calibration curves is determined using at least one of empirical data, and predicted data.
20 . The system of claim 13 , wherein the plurality of first and second calibration curves are associated with different levels of hematocrit.
21 . The system of claim 20 , wherein the different levels of hematocrit include at least one of a high level greater than about 42%, a physiological level of about 42%, and a low level less than about 42%.
22 . The system of claim 13 , further comprising a processor configured to:
measure a third current during a third time-segment following application of the potential excitation;
calculate a plurality of third analyte concentrations based on the third measured current and a plurality of third calibration curves associated with the third time-segment; and
determine a final analyte concentration based on at least one of the first and second analyte concentrations and at least one of the third analyte concentrations.
23 . The system of claim 13 , wherein the analyte is glucose and the fluid sample includes blood.
24 . The system of claim 13 , wherein the fluid sample includes an enzyme of at least one of glucose oxidase and glucose dehydrogenase and a mediator of at least one of potassium ferricyanide and ruthenium hexamine.
25 . The system of claim 13 , wherein the set of electrodes are contained within in a test strip.
26 . The system of claim 13 , wherein the processor is contained within a meter.
27 . A computer readable media, wherein the media comprises a plurality of instructions configured to direct a processor to:
measure a first current during a first time-segment following application of a potential excitation, wherein the potential excitation is applied to a fluid sample containing an analyte;
measure a second current during a second time-segment following application of the potential excitation;
calculate a plurality of first analyte concentrations based on the first measured current and a first set of calibration data associated with the first time-segment; and
calculate a plurality of second analyte concentrations based on the second measured current and a second set of calibration data associated with the second time-segment.
28 . The computer readable media of claim 27 , wherein the instructions further direct the processor to:
determine a final analyte concentration based on at least one of the first analyte concentrations and at least one of the second analyte concentrations.
29 . The computer readable media of claim 28 , wherein the final analyte concentration is determined using at least one of an iterative algorithm, an interpolative algorithm, a line-plot, and an extrapolative algorithm.
30 . The computer readable media of claim 27 , wherein the calibration data includes at least one of a plurality of calibration curves, a lookup table, a data array, and a mathematical equation.
31 . The computer readable media of claim 27 , wherein the first time-segment is less than about two to ten seconds, and the second time-segment is more than about two to ten seconds.
32 . The computer readable media of claim 31 , wherein the first time-segment is less than about eight seconds, and the second time-segment is more than about eight seconds.
33 . The computer readable media of claim 27 , wherein at least part of the calibration data is determined using at least one of empirical data, and predicted data.
34 . The computer readable media of claim 27 , wherein the first and second sets of calibration data are associated with different levels of hematocrit.
35 . The computer readable media of claim 34 , wherein the different levels of hematocrit include at least one of a high level greater than about 42%, a physiological level of about 42%, and a low level less than about 42%.
36 . The computer readable media of claim 27 , wherein the instructions further direct the processor to:
measure a third current during a third time-segment following application of the potential excitation;
calculate a plurality of third analyte concentrations based on the third measured current and a third set of calibration data associated with the third time-segment; and
determine a final analyte concentration based on at least one of the first and second analyte concentrations and at least one of the third analyte concentrations.