Method and apparatus for super montage large area spectroscopic imaging
The disclosure relates to Method and Apparatus for Super Montage Large area Spectroscopic Imaging. In one embodiment of the disclosure, a method for producing a spectroscopic image of an object includes the steps of (a) irradiating the object with light to thereby produce from the object scattered and/or emitted (interchangeably “scattered”) light for each of a plurality of wavelengths; (b) producing separately for each of the plurality of wavelengths a plurality of substantially contiguous sub-images of the object; (c) compensating for spatial aberrations in ones of the sub-images for a select one of the plurality of wavelengths; (d) compensating for intensity aberrations between ones of the substantially contiguous sub-images for one of the plurality of wavelengths; and (e) combining the sub-images for the select one wavelength to thereby produce said spectroscopic image of the object.
1. A method for producing a spectroscopic image of an object comprising the steps of: (a) producing separately for each of the plurality of wavelengths a plurality of substantially contiguous sub-images of the object; (b) compensating for spatial aberrations in ones of the sub-images for a select one of the plurality of wavelengths; (c) compensating for intensity aberrations between ones of the substantially contiguous sub-images for one of the plurality of wavelengths; and (d) combining the sub-images for the select one wavelength to thereby produce said spectroscopic image of the object.
2. The method of claim 1 wherein the spectroscopic image is a Raman image.
3. A method for producing a corrected spectroscopic sub-image of an object comprising the steps of: (a) producing separately for each of the plurality of wavelengths a plurality of substantially contiguous sub-images of the object; (b) compensating for spatial aberrations in ones of the sub-images for a select one of the plurality of wavelengths; and (c) compensating for intensity aberrations between ones of the substantially contiguous sub-images for one of the plurality of wavelengths.
4. The method of claim 3 wherein the spectroscopic sub-image is a Raman image.
5. In a method for reducing aberrations in a spectroscopic image comprising plural substantially contiguous sub-images having plural pixels each pixel having an initial intensity value, where the plural sub-images are each produced using an active optical imaging filter, the improvement comprising the steps of: (a) compensating for spatial aberrations including determining a difference in intensity value between a spatially-filtered mean intensity value and an overall average of the spatially-filtered mean intensity value; and (b) compensating for intensity aberrations.
6. The method of claim 5 wherein the spectroscopic image is a Raman image.
7. In a method for reducing aberrations in a spectroscopic image comprising plural substantially contiguous sub-images having plural pixels each pixel having an initial intensity value, where the plural sub-images are each produced using an active optical imaging filter, the improvement comprising the steps of: (a) compensating for spatial aberrations; and (b) compensating for intensity aberrations including the steps of: (1) normalizing an intensity value for a select group of pixels in one sub-image; and (2) recentering a mean intensity value for the select group of pixels.
8. The method of claim 7 wherein the spectroscopic image is a Raman image.
9. A system for producing a spectroscopic image of an object comprising: circuitry for producing separately for each of a plurality of wavelengths a plurality of substantially contiguous sub-images of the object; circuitry for compensating for spatial aberrations in ones of the sub-images for a select one of the plurality of wavelengths; circuitry for compensating for intensity aberrations between ones of the substantially contiguous sub-images for one of the plurality of wavelengths; and circuitry for combining the sub-images for the select one wavelength to thereby produce said spectroscopic image of the object.
10. The system of claim 9 further comprising a light source for irradiating the object with light to thereby produce from the object scattered or emitted light for each of the plurality of wavelengths.
11. The system of claim 9 wherein each sub-image comprises plural pixels each having an initial intensity value, and said circuitry for compensating for intensity aberrations includes: circuitry for normalizing an intensity value for a select group of pixels in one sub-image as a function of an intensity value for selected corresponding pixels; and circuitry for recentering a mean intensity value for the select group of pixels.
12. The system of claim 9 wherein the spectroscopic image is a Raman image.
13. In a system for reducing aberrations in a spectroscopic image comprising plural substantially contiguous sub-images having plural pixels each pixel having an initial intensity value, where the plural sub-images are each produced using an active optical imaging filter, the improvement comprising: circuitry for compensating for spatial aberrations by determining a difference in intensity value between a spatially-filtered mean intensity value and an overall average of the spatially-filtered mean intensity value; and circuitry for compensating for intensity aberrations.
14. The system of claim 13 wherein the spectroscopic image is a Raman image.
15. In a system for reducing aberrations in a spectroscopic image comprising plural substantially contiguous sub-images having plural pixels each pixel having an initial intensity value, where the plural sub-images are each produced using an active optical imaging filter, the improvement comprising: circuitry for compensating for spatial aberrations; and circuitry for compensating for intensity aberrations by normalizing an intensity value for a select group of pixels in one sub-image and recentering a mean intensity value for the select group of pixels.
16. The system of claim 15 wherein the spectroscopic image is a Raman image.
17. A spectroscope for irradiating an object with light to thereby produce from the object scattered or emitted light for each of a plurality of wavelengths and to produce separately for each of the plurality of wavelengths a plurality of substantially contiguous sub-images of the object, where the spectroscope comprises: circuitry for compensating for spatial aberrations in ones of the sub-images for a select one of the plurality of wavelengths; circuitry for compensating for intensity aberrations between ones of the substantially contiguous sub-images for one of the plurality of wavelengths; and circuitry for combining the sub-images for the select one wavelength to thereby produce said spectroscopic image of the object.
18. A system for producing a corrected spectroscopic sub-image of an object comprising: circuitry for producing separately for each of a plurality of wavelengths a plurality of substantially contiguous sub-images of the object; circuitry for compensating for spatial aberrations in ones of the sub-images for a select one of the plurality of wavelengths; and circuitry for compensating for intensity aberrations between ones of the substantially contiguous sub-images for one of the plurality of wavelengths.
19. The system of claim 18 further comprising a light source for irradiating the object with light to thereby produce from the object scattered or emitted light for each of the plurality of wavelengths.
20. The system of claim 18 wherein each sub-image comprises plural pixels each having an initial intensity value, and said circuitry for compensating for intensity aberrations includes: circuitry for normalizing an intensity value for a select group of pixels in one sub-image as a function of an intensity value for selected corresponding pixels; and circuitry for recentering a mean intensity value for the select group of pixels.
21. The system of claim 18 wherein the spectrographic sub-image is a Raman image.