Integration of sample storage and sample management for life science
Compositions and methods are disclosed for automated storing, tracking, retrieving and analyzing biological samples, including dry storage at ambient temperatures of nucleic acids, proteins (including enzymes), and cells using a dry storage matrix that permits recovery of biologically active materials. RFID-tagged biological sample storage devices featuring dissolvable or dissociable matrices are described for use as supports of biological samples, which matrices can be dried and subsequently rehydrated for sample recovery. Also disclosed are computer-implemented systems and methods for managing sample data.
1. A system for the storage, organization, tracking, retrieval, and analysis of nucleic acid sample, the system comprising:
(i) a biological sample device comprising one or a plurality of wells, each well having disposed therein a matrix for dry storage of the nucleic acid sample without refrigeration and without lyophilization; wherein the matrix comprises at least one stabilizer, wherein if the at least one stabilizer comprises a first stabilizer that is trehalose, then a trehalase inhibitor is also present as a second stabilizer; and wherein the matrix is free of fibers and cellulosic materials and at least 50% of the nucleic acid sample is recovered when an aqueous solvent is added to a the matrix;
(ii) a computer-implemented system for receiving and transmitting data regarding the sample device; and
(iii) a radio frequency interface between the sample device and the computer-implemented system for providing a communication link between the computer-implemented system and the sample device.
2. The system of claim 1 wherein the radio frequency interface comprises a radio frequency interrogator coupled to the computer-implemented system and at least one transponder device associated with the sample storage device for radio frequency communication with the interrogator.
3. The system of claim 1 wherein the biological sample storage device further comprises a magnetic closure and an airtight junction around each at least one well.