Dispensing apparatus and in-duct bubble presence determining method in the same
View Patent ↗A dispensing apparatus performs dispensing by filling a duct to which a dispensing nozzle is connected with a liquid, making the liquid move in the duct to absorb a liquid sample including a specimen or a reagent through the dispensing nozzle, and discharging the absorbed liquid sample; and an in-duct bubble presence determining method in the dispensing apparatus. The presence determining method includes discharging the liquid in the duct from the dispensing nozzle and detecting a transition of a pressure in the duct; calculating the number of mountain pulses in a pressure transition waveform based on the detected pressure transition; and determining a presence of a bubble in the duct based on the calculated number of mountain pulses.
1. An in-duct bubble presence determining method in a dispensing apparatus comprising a dispensing nozzle, a duct, a pressure sensor, and a presence determining unit, which performs dispensing by filling the duct to which the dispensing nozzle is connected with a liquid, making the liquid move in the duct to absorb a liquid sample including one of a specimen and a reagent through the dispensing nozzle, and discharging the absorbed liquid sample, comprising the steps of:
the dispensing nozzle discharging the liquid in the duct;
the pressure sensor detecting a transition of a pressure in the duct at the time of the discharge;
the presence determining unit calculating a number of pulses in a pressure transition waveform based on the detected pressure transition; and
the presence determining unit determining a presence of a bubble in the liquid filling the duct based on the calculated number of pulses.
2. The in-duct bubble presence determining method in the dispensing apparatus according to claim 1 , wherein when the number of pulses which exceed a predetermined threshold value is not more than one, it is determined that the bubble is present in the liquid filling the duct in the step of determining the presence of the bubble.
3. A dispensing apparatus which determines the presence of an in-duct bubble and performs dispensing by filling a duct to which a dispensing nozzle is connected with a liquid, making the liquid move in the duct to absorb a liquid sample including one of a specimen and a reagent through the dispensing nozzle, and discharging the absorbed liquid sample, comprising:
the duct; and
the dispensing nozzle that discharges the liquid in the duct;
a pressure sensor that detects a transition of a pressure in the duct at the time of the discharge; and
a presence determining unit 13 that calculates a number of pulses in a pressure transition waveform based on the pressure transition in the duct detected by the pressure sensor, and determines a presence of a bubble in the liquid filling the duct based on the calculated number of pulses.
4. The dispensing apparatus according to claim 3 , wherein the presence determining unit 13 is configured to determine that the bubble is present in the liquid filling the duct when the number of pulses which exceed the predetermined threshold value is not more than one.