ATM-dependent phosphorylation of Sp1 is involved in the cellular response to DNA damage and enhances cellular survival after DNA damage
View Patent ↗The present invention is related to the discovery that phosphorylation of SP1 (SEQ ID NO.: 2) at serine residue 101 (known herein as phosphoserine 101 Sp1) is an important part of a cell's response to DNA damage. This phosphorylation event is important for subsequent Sp1 localization to a site of DNA damage and is correlated with increased cellular viability in response to DNA damage.
1. An antibody that specifically binds to the polypeptide of SEQ ID NO: 2 having a phosphate moiety at serine residue 101 relative to SEQ ID NO: 2.
2. The antibody of claim 1 , wherein said antibody is a polyclonal antibody, or fragment thereof.
3. The antibody of claim 1 , wherein said antibody is a monoclonal antibody, or fragment thereof.
4. The antibody of claim 1 , wherein said antibody comprises a detectable label.
5. The antibody of claim 4 , wherein said detectable label is selected from the group consisting of a radioactive, a fluorescent, a biological and an enzyme label.