Microarray system with improved sequence specificity
View Patent ↗The invention provides a novel array method for nucleic acid sequence detection with improved specificity which allows for detection of genetic variation, from simple SNPs (where the variation occurs at a fixed position and is of limited allelic number) to more complex sequence variation patterns (such as with multigene families or multiple genetic strains of an organism where the sequence variation between the individual members is neither fixed nor consistent). The array is comprised of short, synthetic oligonucleotide probes attached to a solid surface which are hybridized to single-stranded targets. Single stranded targets can be produced using a method that employs primers modified on the 5′ end to prohibit degradation by a 5′-exonuclease that is introduced to degrade the unprotected strand. The invention further provides for printing buffers/solutions for the immobilization of oligonucleotide probes to an array surface. The invention also provides hybridization and wash buffers and conditions to maximize hybridization specificity and signal intensity, and reduce hybridization times.
1. A method of preparing a detectably labeled single-stranded polynucleotide target from a double stranded DNA comprising the detectably labeled polynucleotide target hybridized to a complementary polynucleotide comprising:
contacting the double stranded DNA with a 5′ to 3′ exonuclease under suitable conditions to degrade at least a portion of the complementary polynucleotide to form a detectably labeled single-stranded polynucleotide target, the polynucleotide target comprising no more than two phosphorothioate bonds and at least one of:
(1) a cyanine dye moiety positioned between the second and third nucleotides from the 5′ end of the polynucleotide target; or
(2) a cyanine dye moiety positioned between first and second nucleotides from the 5′ end of the polynucleotide target and having a modified linkage between the second and third nucleotides from the 5′ end of the polynucleotide target; or
(3) a cyanine dye moiety attached at the 5′ end of the polynucleotide target, with the complementary strand modified with:
(a) a 5′-phosphate group; or
(b) a primary amine with an aliphatic linker arm connected to the polynucleotide via a phosphate linkage and the first 5′-residue of said polynucleotide being an A or a T base; or
(4) a dye moiety attached at the 5′ end of the polynucleotide target and the first 5′-residue of said polynucleotide being a G or C base, with the complementary strand modified with:
(a) a 5′-phosphate group; or
(b) a primary amine with an aliphatic linker arm connected to the polynucleotide via a phosphate linkage and the first 5′-residue of said polynucleotide being an A or a T base.
2. The method of claim 1 , the target polynucleotide comprising a cyanine dye moiety positioned between first and second nucleotides from the 5′ end of the polynucleotide target and having a modified linkage between the second and third nucleotides from the 5′ end of the polynucleotide target, wherein the modified linkage is a nuclease resistant bond.
3. The method of claim 2 , wherein the nuclease resistant bond is a phosphorothioate bond.
4. The method of claim 1 , wherein the polynucleotide target comprises a cyanine dye moiety positioned between the second and third nucleotides from the 5′ end of the polynucleotide target and the 5′-3′ exonuclease is T7 exonuclease.
5. The method of claim 1 , wherein the polynucleotide target comprises a cyanine dye moiety positioned between first and second nucleotides from the 5′ end of the polynucleotide target and has a modified linkage between the second and third nucleotides from the 5′ end of the polynucleotide target, and wherein the 5′-3′ exonuclease is T7 exonuclease.
6. The method of claim 1 , wherein the target polynucleotide comprises a cyanine dye moiety attached at the 5′ end of the polynucleotide target, wherein the complementary strand is modified with a 5′-phosphate group or a primary amine with an aliphatic linker arm connected to the complementary polynucleotide via a phosphate linkage and the first 5′-residue of the complementary polynucleotide is an A or a T base, and wherein the 5′-3′ exonuclease is lambda exonuclease.
7. The method of claim 1 , wherein the target polynucleotide comprises a dye moiety attached at the 5′ end of the polynucleotide target and the first 5′-residue of the target polynucleotide is a G or C base, wherein the complementary strand is modified with a 5′-phosphate group or a primary amine with an aliphatic linker arm connected to the polynucleotide via a phosphate linkage, the first 5′-residue of said polynucleotide being an A or a T base, and wherein the 5′-3′ exonuclease is lambda exonuclease.