Species detection methods and systems
View Patent ↗The disclosure provides methods, systems and kits for cellular and subcellular identification in a rapid, throughput manner.
1. A method of identifying a species of microorganism, comprising:
contacting an intact microorganism with a colorogenic agent, a fluorescent agent, a bioluminescent agent or any combination thereof;
measuring the kinetic change in detected agent over a period of time to provide a kinetic profile; and
comparing the kinetic profile to a known profile, wherein a similarity in kinetic profile to known profile is indicative of the species of microorganism.
2. The method of claim 1 , wherein the intact microorganism is a bacteria.
3. The method of claim 2 , wherein the bacteria is gram negative.
4. The method of claim 1 , wherein the intact microorganism is a eukaryotic cell.
5. The method of claim 1 , wherein the fluorescent agent is a dye set forth in FIG. 1 .
6. The method of claim 1 , wherein the method is carried out in a microfluidic device.