Injectable and swellable microspheres for tissue bulking
The present invention relates to injectable compositions comprising biocompatible, swellable, hydrophilic, non-toxic and substantially spherical microspheres useful for tissue bulking. The invention also relates to methods of tissue bulking, particularly for the treatment of Gastro-esophageal reflux disease, urinary incontinence, or urinary reflux disease, using the injectable compositions.
1. A method of tissue bulking in a mammal for the treatment of gastroesophageal reflux disease, comprising injecting a composition comprising swellable, biocompatible, hydrophilic, non-toxic and substantially spherical microspheres, having a diameter of about 100 microns to about 300 microns prior to injection, in a biocompatible carrier to said mammal through a needle of about 18 to 26 gauge, wherein the microspheres are non-aggregating and do not clump together, and wherein the microspheres comprise sodium acrylate polymer, acrylamide polymer, acrylamide derivative polymer or copolymer, sodium acrylate and vinyl alcohol copolymer, or mixtures thereof.
2. The method of claim 1 , wherein the composition is a suspension of said microspheres in said biocompatible carrier.
3. The method of claim 1 , wherein the microspheres swell upon contact with physiological fluids at the injection site.
4. The method of claim 1 , wherein diameters of the microspheres after injection are about 1 to about 4 times of diameters of the microspheres immediately prior to injection.
5. The method of claim 1 , wherein the polymers comprise from about 0.5% to about 20%, by molecular weight, of crosslinkers.
6. The method of claim 1 , which further comprises cells associated with surfaces of at least a portion of the microspheres prior to administration.
7. The method of claim 6 , wherein the cells are autologous cells from the subject mammal.
8. The method of claim 7 , wherein the autologous cells are fat cells, muscle cells, subcutaneous cells, dermal cells, epidermal cells, or combinations thereof.
9. The method of claim 2 , wherein the biocompatible carrier is an emulsion.
10. The method of claim 2 , wherein the biocompatible carrier is an organic or a non-aqueous solvent.
11. The method of claim 2 , wherein the biocompatible carrier is an aqueous solution, a hydro-organic solution, or mixtures thereof.
12. The method of claim 2 , wherein the biocompatible carrier comprises salts composed of cations selected from the group consisting of sodium, potassium, calcium, magnesium, iron, zinc, and ammonium in an amount of from about 0.01 M to about 5 M.
13. The method of claim 12 , wherein the salt is supplied in the form of a contrast agent.
14. The method of claim 2 , wherein the biocompatible carrier is acylamino-e-propion-amido-3-triiodo-2,4,6 benzoic acid.
15. The method of claim 1 , wherein the composition further comprises a therapeutic agent, a radio-pacifying agent, a contrast media, or mixtures thereof.
16. The method of claim 15 , wherein said therapeutic agent is bound to the microspheres.
17. The method of claim 1 , wherein the injection is into an area of said mammal in need of tissue bulking.
18. The method of claim 1 , wherein said composition is injected into a lower esophageal sphincter or diaphragm of said mammal.
19. The method of claim 1 , wherein the mammal is a human.
20. The method of claim 1 , wherein the administration comprises injecting said composition extracorporeally into organs, components of organs, or tissues prior to their inclusion into said mammal's body, organs, or components of organs.
21. The method of claim 1 , wherein the needle has a gauge of about 22 to about 24.
22. The method of claim 1 , wherein the biocompatible carrier comprises saline.
23. A method of tissue bulking in a mammal for the treatment of gastroesophageal reflux disease, comprising injecting a composition comprising swellable, biocompatible, hydrophilic, non-toxic and substantially spherical microspheres, having a diameter of about 100 microns to about 300 microns prior to injection, in a biocompatible carrier to said mammal through a needle of about 18-26 gauge, wherein said microspheres comprise a sodium acrylate and vinyl alcohol copolymer.
24. The method of claim 23 , wherein the administration comprises injecting said composition into the lower esophageal sphincter or the diaphragm of said mammal.
25. The method of claim 23 , wherein diameters of the microspheres after injection are about 1 to about 4 times of diameters of the microspheres immediately prior to injection.
26. A method of tissue bulking in a mammal for the treatment of gastroesophageal reflux disease, comprising injecting a composition comprising swellable, biocompatible, hydrophilic, non-toxic and substantially spherical microspheres, having a diameter of about 100 microns to about 300 microns prior to injection, in a biocompatible carrier to said mammal through a needle of about 18 to 26 gauge, wherein the microspheres comprise sodium acrylate and vinyl alcohol copolymer, N-tris-hydroxymethyl methylacrylamide polymer, diethylaminoethylacrylamide polymer, or N,N-methylene-bis-acrylamide polymer, or mixtures thereof.