Generation of plants with improved drought tolerance
View Patent ↗The present invention is directed to plants that display a drought tolerance phenotype due to altered expression of a DR02 nucleic acid. The invention is further directed to methods of generating plants with a drought tolerance phenotype.
1. A method, comprising:
identifying a plant with defective endogenous DRO2 activity;
transforming the plant having defective endogenous DRO2 activity with a plant transformation vector comprising a heterologous constitutive promoter operatively linked to a nucleotide sequence that encodes a DRO2 polypeptide comprising an amino acid sequence having at least 95% sequence identity to the amino acid sequence of SEQ ID NO:2, wherein the heterologous constitutive promoter provides overexpression of a DRO2 transcript encoding said DRO2 polypeptide;
subjecting the transformed plant to water deprivation; and
measuring the relative water content of the transformed plant, wherein an increase in relative water content of the transformed plant as compared to a control plant which did not receive the plant transformation vector indicates that the transformed plant has increased drought tolerance, and thereby imparting drought tolerance to the plant which had defective endogenous DRO2 activity prior to said transformation.
2. The method of claim 1 , wherein subjecting the transformed plant to water deprivation comprises depriving the transformed plant of water for at least 10 days.
3. The method of claim 1 , wherein the polypeptide consists of an amino acid sequence having at least 95% sequence identity with SEQ ID NO:2.
4. The method of claim 1 , wherein the polypeptide comprises the amino acid sequence set forth in SEQ ID NO:2.
5. The method of claim 1 , wherein the polypeptide consists of the amino acid sequence set forth in SEQ ID NO:2.