Fluorescence resonance energy transfer detection with nanoparticles for in vitro and in vivo applications
View Patent ↗A combination of nanoparticles is disclosed comprised of amine functionalized polyethylene glycol in which one particle with a fluorescent donor dye having one wavelength excitation maximum and at least one additional particle with a second fluorescent dye having a second, higher wavelength excitation maximum, the particles having the same or different biomolecule targeting moieties bound to their external surfaces.
1. A method for visualizing close proximity of biomolecules in a specimen, comprising:
treating the specimen with at least one FRET particle pair comprising two or more fluorescent particles, each fluorescent particle composed of a cross-linked polymer with 30-50 weight percent of the monomer of Formula 1
where n is 10 to 200,
at least one of the fluorescent particles including an energy donor dye and at least one other of the fluorescent particles including an energy acceptor dye, wherein the energy donor dye and energy acceptor dye are dispersed within the fluorescent particles, and each fluorescent particle including one or more targeting moieties covalently attached to an external surface;
exposing the specimen to a light source and recording emitted light with a detector; and
visualizing the close proximity of the biomolecules in the specimen.
2. The method of claim 1 wherein the fluorescent particles comprise at least about 5 weight percent covalently bound polyethylene glycol.
3. The method of claim 1 wherein the energy donor dye and energy acceptor dye are randomly distributed within the fluorescent particles.
4. The method of claim 1 wherein the particles are 10 to 100 nm in size.
5. The method of claim 1 , wherein the particles include methoxymethyl methacrylate at 45% or less by weight as co-monomer.
6. The method of claim 1 , wherein the energy acceptor and energy donor dyes are hydrophobic dyes having an absorbance maximum between 500 and 900 nm.
7. The method of claim 1 , wherein the particles include at least on dye selected from the group consisting of:
8. The method of claim 1 , wherein the targeting moieties are selected from the group consisting of antibodies, affinity ligands, peptides, proteins, pharmaceuticals, oligonucleotides and combinations thereof.