Compositions containing LAG-3 and cells that secrete GM-CSF and methods of use
View Patent ↗The present invention relates to a method of enhancing anti-tumor protection in a mammal. More particularly, the invention is concerned with combinations comprising antigen presenting cell (APC) activators and a cytokine-secreting cell and methods of administering the combination for enhanced immune response to tumor cells in a patient with a cancer.
1. A composition for stimulating an immune response to cancer in a subject, comprising a synergistic combination of:
a) an allogeneic tumor cell line comprising cells that express an antigen of the cancer and that express GM-CSF and which cells have been rendered proliferation-incompetent and
b) an APC activator which is LAG-3.
2. The composition of claim 1 , wherein the cells of said cell line are rendered proliferation-incompetent by irradiation.
3. The composition of claim 1 , wherein said allogeneic cell line is a tumor cell line selected from the group consisting of a prostate tumor line, a non-small cell lung carcinoma line and a pancreatic cancer line.
4. The composition of claim 1 , wherein said LAG-3 is expressed by cells autologous to said subject, or allogeneic to said subject, or are bystander cells.
5. The composition of claim 4 , wherein the cells that express LAG-3 are rendered proliferation-incompetent by irradiation.
6. A method for stimulating an immune response to cancer in a subject which comprises administering to a subject in need of such stimulating an effective amount of the composition of claim 1 .