Composition and method for treating bacterial, viral, fungal diseases, inflammation and pain
View Patent ↗The present invention is directed to compositions and methods of treating bacterial, viral, fungal diseases; inflammation or inflammatory-related disorders; pain; and skin conditions. The composition comprises an organic solvent extract, which is prepared by the method comprising the steps of: (a) mixing methionine with water, (b) adding an aqueous hypochlorite solution to the methionine solution and mixing, (c) adding a water-immiscible organic solvent to (b) and mixing, and (d) separating the organic solvent phase from the water phase to obtain the organic solvent extract. The present invention is also directed to a method for preparing the organic solvent extract.
1. A method for preparing an organic solvent extract comprising the steps of:
(a) mixing methionine with water,
(b) adding an aqueous hypochlorite solution to (a) and mixing at a temperature between 0° C. to ambient temperature,
(c) adding a water-immiscible organic solvent to (b) and mixing, and
(d) separating the organic solvent phase from the aqueous phase.
2. The method according to claim 1 , wherein said water-immiscible organic solvent is hexane, heptane, methylene chloride, mineral oil, vegetable oil, or ethyl acetate.
3. The method according to claim 2 , wherein said water-immiscible organic solvent is mineral oil or hexane.
4. The method according to claim 2 , wherein said water-immiscible organic solvent is ethyl acetate.
5. The method according to claim 1 , wherein the aqueous hypochlorite solution is an aqueous sodium hypochlorite solution.
6. The method extract according to claim 1 , wherein the step (b) is carried out under inert gas.
7. The method according to claim 1 , wherein the methionine is D-methionine, L-methionine, or a mixture thereof.
8. The method according to claim 1 , further comprising the step of removing non-soluble residues after separating the organic solvent phase.
9. The method according to claim 8 , wherein the non-soluble residues is removed by filtration, decanting, or centrifugation.
10. The method according to claim 1 , wherein the mixing in step (b) is carried out at a temperature between 0° C. to 5° C.
11. The method according to claim 1 , wherein step (c) is carried out within 5 minutes after step (b).
12. An organic solvent extract prepared according to the method of claim 1 .
13. A method of treating skin conditions in a subject comprising the step of topically administering a composition comprising the organic solvent extract of claim 12 to the afflicted area of skin in a subject, wherein said skin conditions are skin damages by bums, skin damages by sun, skin blotches, or wart.