Vascular-preferred promoters
View Patent ↗The present invention relates to the regulation of polynucleotide transcription and/or expression. In particular, this invention relates to polynucleotide regulatory sequences isolated from Eucalyptus grandis and Pinus radiata that are capable of conferring vascular-preferred polynucleotide transcription in plant cells. Constructs and methods for using the inventive regulatory sequences for modifying transcription of endogenous and/or heterologous polynucleotides also are included in the invention.
1. An isolated nucleic acid molecule comprising the sequence of SEQ ID NO: 7; wherein said sequence is a Ferulate-5-Hydroxylase promoter conferring xylem-preferred gene expression in a plant cell.
2. The isolated nucleic acid molecule of claim 1 , wherein said sequence is operably linked to a gene encoding an RNA interference molecule.
3. A vector comprising the isolated nucleic acid molecule of claim 1 , wherein said sequence is operably linked to a gene encoding an RNA interference molecule.
4. A plant cell transformed with the vector of claim 3 .
5. A transgenic plant comprising the plant cell of claim 4 , wherein the transgenic plant expresses the gene encoding the RNA interference molecule.
6. The transgenic plant of claim 5 , wherein the transgenic plant is selected from angiosperms and gymnosperms.
7. A method for regulating the lignin content of a plant, comprising (a) transforming a plant cell with a vector comprising the isolated nucleic acid molecule of claim 1 operably linked to a gene encoding an enzyme involved in lignin biosynthesis; (b) culturing the transformed plant cell under conditions that promote growth of a plant; (c) selecting a transgenic plant; and (d) measuring the lignin content in the transgenic plant, wherein said transgenic plant has reduced lignin content compared to a non-transgenic plant of the same species.
8. A method for obtaining wood with a reduced lignin content, comprising (a) transforming a woody plant cell with a vector comprising the isolated nucleic acid molecule of claim 1 operably linked to a gene that encodes an enzyme involved in lignin biosynthesis; (b) culturing the transformed plant cell under conditions that promote growth of a woody plant; (c) selecting a transgenic woody plant; and (d) obtaining wood from the transgenic woody plant, wherein the wood obtained from the transgenic woody plant has a reduced lignin content compared to the wood obtained from a non-transgenic woody plant of the same species.
9. The method of claim 8 , wherein the woody plant is a species of Eucalyptus or Pinus.
10. The method of claim 8 , further comprising the step of making wood pulp from the wood obtained from the transgenic woody plant.
11. The method of claim 10 , wherein the woody plant is a species of Eucalyptus or Pinus.
12. The method of claim 8 , further comprising the step of making paper from the wood obtained from the transgenic woody plant.
13. The method of claim 12 , wherein the woody plant is a species of Eucalyptus or Pinus.
14. A gene operably linked to the isolated nucleic acid molecule of claim 1 , wherein said sequence regulates the expression of the operably-linked gene in a plant.
15. A DNA construct comprising the isolated nucleic acid molecule of claim 1 operably linked to a nucleic acid sequence, wherein said isolated nucleic acid molecule regulates the expression of the operably linked nucleic acid sequence in a plant.
16. A plant cell comprising the DNA construct of claim 15 .
17. A transgenic plant comprising the plant cell of claim 16 .
18. A method of regulating the composition or content of lignin in a plant comprising (a) introducing into a plant cell the DNA construct of claim 15 , wherein the operably linked nucleic acid sequence encodes an enzyme involved in lignin biosynthesis; (b) culturing the plant cells under conditions that promote growth of a plant; and (c) selecting a transgenic plant that has a lignin composition or content that is different from the lignin composition or content of a plant of the same species that is not transformed with the DNA construct.