Methods of producing organic products with photosynthetic organisms
View Patent ↗Provided herein are compositions and methods for producing products by photosynthetic organisms. The photosynthetic organisms can be genetically modified to effect production, expression, or both, of products. The methods and compositions are particularly useful in the petrochemical industry.
1. A method of generating a fuel product from an aquatic non-vascular C 3 photosynthetic organism comprising:
a. growing an aquatic non-vascular C 3 photosynthetic organism in an excess of fossil fuel inorganic carbons such that carbon fixation during growth within said aquatic non-vascular C 3 photosynthetic organism is not limited by the availability of inorganic carbons, wherein said aquatic non-vascular C 3 photosynthetic organism generates a first fuel product; and
b. incorporating carbons from said fossil fuel inorganic carbons into said first fuel product, wherein said first fuel product has a δ 13 C distribution of less than −40‰.
2. The method of claim 1 , wherein said fossil fuel inorganic carbons comprises carbon dioxide comprising 13 C and carbon dioxide comprising 12 C.
3. The method of claim 1 , wherein said aquatic non-vascular C 3 photosynthetic organism comprises one or more nucleic acids encoding one or more enzymes whose end product is said first fuel product.
4. The method of claim 3 , wherein said nucleic acids are heterologous.
5. The method of claim 1 , wherein said first fuel product is not naturally produced by said aquatic non-vascular C 3 photosynthetic organism.
6. The method of claim 1 , wherein said first fuel product comprises a terpene or terpenoid.
7. The method of claim 1 , wherein said fossil fuel inorganic carbons have a δ 13 C distribution of greater than −32‰.
8. The method of claim 1 , further comprising extracting said first fuel product.
9. The method of claim 8 , further comprising refining said first fuel product.
10. The method of claim 9 , wherein said refining comprises at least one of the processes selected from the group consisting of the following: hydrocracking, catalytic cracking, steam cracking, cracking, fractionating, distilling, hydrotreating, and any combination thereof.
11. The method of claim 8 , further comprising creating a second fuel product comprising said first fuel product and a fuel component.
12. The method of claim 1 , further comprising combusting said first fuel product and thus generating δ 13 C enriched inorganic carbons.
13. The method of claim 12 , wherein said δ 13 enriched inorganic carbons have a δ 13 C distribution of less than −40‰.
14. The method of claim 1 , wherein said first fuel product has a δ 13 C distribution of less than −45‰.
15. The method of claim 1 , wherein said first fuel product has a δ 13 C distribution of less than −50‰.
16. The method of claim 1 , wherein said first fuel product has a δ 13 C distribution of less than −55‰.
17. The method of claim 1 , wherein said first fuel product has a δ 13 C distribution of less than −60‰.
18. The method of claim 1 , wherein said aquatic non-vascular C 3 photosynthetic organism is an alga.
19. The method of claim 1 , wherein said aquatic non-vascular C 3 photosynthetic organism is a cyanobacterium.