IP Library Granted Patent US 7,858,371
Granted Patent B2
US 7,858,371 · App. 12/212,094 · Granted Dec 28, 2010

Method of separating embryo suspension mass

View Patent ↗
Loading inventors, assignments & file history…
Monitor This Case
Get email alerts when status or documents change.
Order Certified Copies
Most orders are placed with the USPTO same day — all within 24 business hours.
Order via The Patent Place →
Pre-filled with this patent's details
Quick Facts
Patent No.
US 7,858,371
App. No.
12/212,094
Granted
Dec 28, 2010
Kind
B2
Abstract

A method of separating an embryo suspension mass is provided. The method includes supplying an embryo suspension mass culture having a plurality of first particles of a first size and a plurality of second particles of a second size different at least in part from the first size of the first particles. The method also includes suspending the embryo suspension mass culture in a fluid to create a mixture and forcing the mixture through a filter while maintaining the mixture in the fluid to separate the first particles from the second particles.

Claims (19)

1. A method of separating an embryo suspension mass, the method comprising:

(a) supplying an embryo suspension mass culture having a plurality of first particles of a first size and a plurality of second particles of a second size different at least in part from the first size of the first particles;

(b) suspending the embryo suspension mass culture in a fluid to create a mixture; and

(c) forcing the mixture through at least a first filter while maintaining the mixture in the fluid to separate the first particles from the second particles.

2. The method of claim 1 wherein forcing the mixture through at least a first filter includes applying a centrifugal force to the mixture while maintaining the mixture in the fluid.

3. The method of claim 1 , wherein suspending the embryo suspension mass culture includes depositing the embryo suspension mass culture into a basket having a plurality of pores sized to permit at least the second particles but not the first particles to pass through the plurality of pores.

4. The method of claim 3 , wherein forcing the mixture through at least a first filter includes applying a rotating motion to the basket to force at least the second particles through the plurality of pores while restraining at least the first particles within the basket.

5. A method of separating an embryo suspension mass, the method comprising:

(a) supplying an embryo suspension mass culture having a plurality of first particles of a first size and a plurality of second particles of a second size different at least in part from the first size of the first particles;

(b) suspending the embryo suspension mass culture in a fluid to create a mixture; and

(c) applying a rotary motion to the mixture to force the mixture through a filter to separate the first particles from the second particles.

6. The method of claim 5 , wherein applying a rotary motion to the mixture includes applying a centrifugal force to a filter holding the mixture.

7. The method of claim 5 , wherein the filter is a basket having a plurality of pores sized to filter at least the first particles from the second particles.

8. The method of claim 7 , wherein applying a rotary motion to the mixture includes applying a centrifugal force to the basket.

9. A method of separating an embryo suspension mass, the method comprising:

(a) supplying an embryo suspension mass culture having a plurality of first particles of a first size and a plurality of second particles of a second size different at least in part from the first size of the first particles;

(b) depositing the embryo suspension mass culture in a basket having a plurality of pores sized to filter at least the first particles from the second particles;

(c) suspending the basket containing the embryo suspension mass culture in a fluid to create a mixture; and

(d) applying a rotary motion to the mixture to force the mixture through the plurality of pores of the basket to separate the first particles from the second particles.

Assignments (2)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Apr 21, 2009
From: WEYERHAEUSER COMPANY
To: WEYERHAEUSER NR COMPANY
Reel/Frame 022835/0233 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Apr 13, 2009
From: PALTIEL-ZUCATI, JUDITH N; STAR, ROBERT A
To: WEYERHAEUSER NR COMPANY
Reel/Frame 022538/0155 →