Methods and compositions for detecting colon cancers
View Patent ↗This application describes methods and compositions for detecting and treating vimentin-associated neoplasia. Differential methylation of the vimentin nucleotide sequences has been observed in vimentin-associated neoplasia such as colon neoplasia.
1. A kit for detecting a vimentin-associated neoplasia in a subject, comprising at least a primer pair, wherein said primer pair is selected from the group of primer pairs consisting of:
a) SEQ ID NOs: 62 and 63
b) SEQ ID NOs: 72 and 71
c) SEQ ID NOs: 23 and 24
d) SEQ ID NOs: 27 and 65
e) SEQ ID NOs: 39 and 64
f) SEQ ID NOs: 54 and 15; and
g) SEQ ID NOs: 56 and 15.
2. The kit of claim 1 , further comprising a compound to convert a template DNA.
3. The kit of claim 2 , wherein the compound is bisulfite.
4. The kit of claim 3 , wherein each primer comprises at least a CpG dinucleotide.
5. A pair of oligonucleotide primers for assessing methylation in the human vimentin gene, wherein said primer pair is selected from the group of primer pairs consisting of:
a) SEQ ID NOs: 62 and 63
b) SEQ ID NOs: 72 and 71
c) SEQ ID NOs: 23 and 24
d) SEQ ID NOs: 27 and 65
e) SEQ ID NOs: 39 and 64
f) SEQ ID NOs: 54 and 15; and
g) SEQ ID NOs: 56 and 15.
6. The primer pair of claim 5 , wherein the primer pair is selected from the group of primer pairs consisting of:
a) SEQ ID NOs: 62 and 63
b) SEQ ID NOs: 72 and 71
c) SEQ ID NOs: 23 and 24
d) SEQ ID NOs: 27 and 65, and
e) SEQ ID NOs: 39 and 64.
7. The primer pair of claim 5 , wherein at least one of the primers in said primer pair is further labeled with a detectable marker.
8. The primer pair of claim 7 , wherein at least one of the primers in said primer pair is labeled with a fluorescent dye.
9. The kit of claim 1 , wherein said kit comprises a primer pair that is selected from the group consisting of:
a) SEQ ID NOs: 62 and 63
b) SEQ ID NOs: 72 and 71
c) SEQ ID NOs: 23 and 24
d) SEQ ID NOs: 27 and 65, and
e) SEQ ID NOs: 39 and 64.
10. The kit of claim 1 , wherein said kit comprises a primer pair that is selected from the group of primer pairs consisting of:
a) SEQ ID NOs: 62 and 63, and
b) SEQ ID NOs: 72 and 71.
11. The oligonucleotide primer pair of claim 5 , wherein the primer pair is selected from the group consisting of:
a) SEQ ID NOs: 62 and 63, and
b) SEQ ID NOs: 72 and 71.