Method of treating or preventing an IL-1 related disease or condition
View Patent ↗Methods of treating or preventing an IL-1 related disease or condition in a mammal comprising administering an effective amount of an IL-1β binding antibody or IL-1β binding fragment thereof. An IL-1β binding antibody or IL-1β binding fragment thereof is provided comprising the amino acid sequence of SEQ ID NO:28, and related nucleic acids, vectors, cells, and compositions, and a method of preparing an affinity matured IL-1β binding polypeptide is provided. IL-1β binding antibodies or IL-1β binding fragments thereof are provided which have desirable affinity and potency.
1. An isolated nucleic acid comprising a nucleic acid sequence that encodes a heavy chain variable region of an IL-1β binding antibody or IL-1β binding fragment thereof, wherein the heavy chain variable region comprises the amino acid sequence of SEQ ID NO: 15.
2. An isolated nucleic acid comprising a nucleic acid sequence that encodes a light chain variable region of an IL-1β binding antibody or IL-1β binding fragment thereof, wherein the light chain variable region comprises the amino acid sequence of SEQ ID NO: 11.
3. An isolated nucleic acid encoding an IL-1β binding antibody or IL-1β binding fragment thereof, wherein the antibody or fragment comprises a heavy chain variable region of SEQ ID NO: 15 and a light chain variable region of SEQ ID NO: 11.
4. An isolated nucleic acid encoding an IL-1β binding antibody or IL-1β binding fragment thereof, wherein the antibody or fragment binds to human IL-1β with a dissociation constant less than 1 pM, and the antibody or fragment competes with the binding of an antibody comprising the heavy chain variable region of SEQ ID NO: 15 and the light chain variable region of SEQ ID NO: 11.
5. An isolated vector comprising the nucleic acid of any one of claims 1 - 4 .
6. An isolated cell comprising the nucleic acid of any one of claims 1 - 4 or the vector of claim 5 .
7. The isolated cell of claim 6 , wherein a first nucleic acid or a first vector encodes the heavy chain variable region and a second nucleic acid or a second vector encodes the light chain variable region.
8. The isolated cell of claim 6 , wherein the heavy chain variable region and the light chain variable region are encoded by the same nucleic acid or the same vector.
9. The isolated cell of claim 6 , wherein said cell is a eukaryotic cell.
10. The isolated cell of claim 9 , wherein said cell is a mammalian cell.
11. The isolated cell of claim 10 , wherein said cell is a Chinese hamster ovary (CHO) cell, NS0 myeloma cell, human embryonic kidney (HEK) 293 cell, COS cell, PER.C6 cell, or SP2 cell.
12. The isolated cell of claim 6 , wherein said cell is a bacterial cell.