Circovirus sequences associated with piglet weight loss disease (PWD)
View Patent ↗The genome sequences and the nucleotide sequences coding for the PWD circovirus polypeptides, such as the circovirus structural and non-structural polypeptides, vectors including the sequences, and cells and animals transformed by the vectors are provided. Methods for detecting the nucleic acids or polypeptides, and kits for diagnosing infection by a PWD circovirus, also are provided. Method for selecting compounds capable of modulating the viral infection is further provided. Pharmaceutical, including vaccines, compositions for preventing and/or treating viral infections caused by PWD circovirus and the use of vectors for preventing and/or treating diseases also are provided.
1. A vaccine for protecting piglets from porcine circovirus type B (PCVB) comprising an ORF′2 protein of PCVB and an acceptable pharmaceutical vehicle.
2. The vaccine according to claim 1 , wherein the ORF′2 protein is a recombinant protein.
3. The vaccine according to claim 1 , wherein the ORF′2 protein is produced by way of expression from a baculovirus expression vector in insect cells, said baculovirus expression vector containing the PCVB ORF′2 gene sequence under control of a suitable promoter.
4. The vaccine according to any one of claims 1 , 2 , and 3 , further comprising a suitable adjuvant.
5. The vaccine according to any one of claims 1 , 2 , and 3 , further comprising a suitable adjuvant, wherein said suitable adjuvant is an oil in water emulsion.
6. A vaccine against porcine circovirus type B (PCVB) comprising an ORF′2 protein of PCVB and an acceptable pharmaceutical vehicle, wherein said ORF′2 protein has at least 90% identity to SEQ ID NO:26.
7. A vaccine against porcine circovirus type B (PCVB) comprising an ORF′2 protein of PCVB and an acceptable pharmaceutical vehicle, wherein said ORF′2 protein is encoded by a nucleic acid having at least 90% identity to SEQ ID NO:25.
8. The vaccine according to claim 6 or claim 7 , further comprising a suitable adjuvant.
9. A method for the protection of piglets against porcine circovirus type B (PCVB) comprising administering the vaccine of claim 1 , claim 6 , or claim 7 .
10. The method according to claim 9 , wherein the ORF′2 protein is a recombinant protein.
11. The method according to claim 9 , wherein the ORF′2 protein is produced by way of expression from a baculovirus expression vector in insect cells, said baculovirus expression vector containing the PCVB ORF′2 gene sequence under control of a suitable promoter.
12. The method according to claim 9 , wherein the vaccine further comprises a suitable adjuvant.
13. The method according to claim 12 , wherein said adjuvant is an oil in water emulsion.
14. The method according to claim 9 , wherein about 0.1 to about 10 μg of said ORF′2 protein per kilogram weight of said piglet is administered per administration.
15. The method according to claim 9 , wherein about 0.2 to about 5 μg of said ORF′2 protein per kilogram weight of said piglet is administered per administration.
16. The method according to claim 9 , wherein about 0.5 to about 2 μg of said ORF′2 protein per kilogram weight of said piglet is administered per administration.
17. The method according to claim 9 , wherein said vaccine is administered more than one time.
18. The method according to claim 9 , wherein said vaccine is administered more than one time and wherein the first and second administrations are staggered over time.