Collision cell for mass spectrometer
View Patent ↗A novel curved collision cell for a mass spectrometer is described. The collision cell includes a straight section having a length that is selected to cause a precursor ion entering the straight section to lose a desired amount of kinetic energy such that when the precursor ion enters the curved section of the collision cell the precursor ion will tend to neither escape nor contact the collision cell, and thereby tending to survive its passage within the curved portion.
1. A collision cell comprising at least one electrode and also comprising:
a straight section having an inlet for receiving a precursor ion at a first end; said straight section configured to, at least one of, allow fragmentation of said precursor ion to generate product ions, allow said precursor ion to lose kinetic energy as it passes through said straight section from a first to a second end and allow said product ions to lose kinetic energy as it passes through said straight section from the first to the second end;
a curved section downstream of the second end of the straight section; the curved section configured to allow fragmentation of said precursor ion and to generate product ions therefrom;
wherein the straight section is about twenty-five millimeters to four centimeters in length, and the curved section has a mean radius of curvature of about forty-five millimeters to about fifty millimeters along its longitudinal axis.
2. The collision cell of claim 1 wherein said collision cell comprises a quadrupole set.
3. The collision cell of claim 1 wherein the straight section and the curved section are mated.
4. The collision cell of claim 1 wherein an intermediate section is disposed between the straight section and the curved section.
5. A method of fabricating a collision cell comprising:
selecting a precursor ion;
determining parameters of a curved section for said collision cell including a desired radius, axial distance, number and configuration of electrodes, operating pressure, and operating frequency in order to generate product ions from said precursor ions;
determining a first level of kinetic energy that would cause said precursor ion to crash into one of said electrodes when said precursor ion is introduced into said collision cell at said first level;
determining a second level of kinetic energy that would cause said precursor ion to survive passage through said curved section when said precursor ion is introduced into said collision cell at said second level;
selecting a length for a straight section of the collision cell to be connected to said curved section; said length being based on a span needed to allow said precursor ion to lose a third level of kinetic energy being substantially equal to a difference between said first level and said second level during travel along said span;
wherein said number and configuration of electrodes are selected to provide a collision cell comprising at least one quadrupole set; and
wherein said straight section is about twenty five millimeters to about four centimeters in length and wherein said curved portion has a mean radius of curvature of about forty-five millimeters to about fifty millimeters along its longitudinal axis.
6. The method of claim 5 further comprising the steps of:
constructing said collision cell with:
said straight section having said length and an inlet for receiving said precursor ion; said straight section for allowing said precursor ion to lose kinetic energy as it passes through said straight section;
a curved section merging at a first end of said curved section with said straight portion and an end of said straight section opposite from said inlet; said curved section for allowing collisions of said precursor ion to generate said product ions therefrom.
7. The method of claim 5 wherein said step of determining said first level of kinetic energy is based on a model for calculating the amount of kinetic energy of said precursor ion as a function of said axial distance and said pressure.
8. The method of claim 5 wherein said step of determining said second level is based on determining an amount of energy needed to confine within said precursor ion within said curved section, whereby said precursor ion's kinetic energy perpendicular to an axis of said curved section is less than a pseudo-potential well depth of said electrodes.
9. A collision cell for a mass spectrometer comprising a curved section and a straight section joined at an entrance to said curved portion; said straight section having a length that is about twenty-five millimeters to four centimeters to cause an ion entering the straight section to lose a desired amount of kinetic energy such that when said ion enters said curved section said ion will neither escape nor contact the collision cell, and thereby survive passage within said curved portion, wherein said curved section has a mean radius of curvature of about forty-five millimeters to about fifty millimeters along its longitudinal axis.
10. A mass spectrometer comprising at least two of quadrupole regions interconnected by a collision cell comprising a curved section and a straight section connected at an entrance to said curved section; said straight section having a length that is about twenty-five millimeters to four centimeters to cause an ion entering the straight section to lose a desired amount of kinetic energy such that when said ion enters said curved section said ion will neither escape nor contact the collision cell, and thereby survive passage within said curved portion, wherein said curved section has a mean radius of curvature of about forty-five millimeters to about fifty millimeters along its longitudinal axis.