Herbicide tolerant cotton plants and methods for producing and identifying same
View Patent ↗The invention pertains to transgenic cotton plants, plant material and seeds, characterized by harboring a specific transformation event, particularly by the presence of a gene encoding a protein that confers herbicide tolerance, at a specific location in the cotton genome. The cotton plants of the invention combine the herbicide tolerant phenotype with optimal agronomic performance.
1. A kit for identifying elite event EE-GH1 in biological samples comprising a first PCR primer comprising a nucleotide sequence of 15 to 35 contiguous nucleotides from the complement of SEQ ID NO:4 from nucleotide 180 to nucleotide 426 and a second PCR primer comprising a nucleotide sequence of 15 to 35 contiguous nucleotides from the nucleotide sequence of SEQ ID NO: 6 from nucleotide 198 to nucleotide 2544 or the complement thereof or from the nucleotide sequence of SEQ ID NO:4 from nucleotide 1 to nucleotide 179.
2. The kit of claim 1 , wherein said first PCR primer comprises a nucleotide sequence of 15 to 35 contiguous nucleotides from the complement of SEQ ID NO:4 from nucleotide 180 to nucleotide 426 and said second PCR primer comprises a nucleotide sequence of 15 to 35 contiguous nucleotides from the nucleotide sequence of SEQ ID NO:4 from nucleotide 1 to nucleotide 179.
3. A kit for identifying elite event EE-GH1 in biological samples comprising a probe comprising a sequence of 15 to 500 contiguous nucleotides which is identical to or complementary to the sequence of plant DNA and foreign DNA at each site contiguous with the insertion site contained in SEQ ID NO: 4.
4. A method for identifying elite event EE-GH1 in biological samples comprising detecting an EE-GH1 specific region with the kit of claim 3 .
5. A method for identifying elite event EE-GH1 in biological samples comprising amplifying a DNA fragment of between 100 and 350 by from a nucleic acid present in said biological samples using a polymerase chain reaction with a kit according to claim 1 .
6. The method of claim 5 using a kit wherein said first PCR primer comprises a nucleotide sequence of 15 to 35 contiguous nucleotides from the complement of SEQ ID NO:4 from nucleotide 180 to nucleotide 426 and said second PCR primer comprises a nucleotide sequence of 15 to 35 contiguous nucleotides from the nucleotide sequence of SEQ ID NO:4 from nucleotide 1 to nucleotide 179.
7. A method for confirming seed purity comprises detecting an EE-GH1 specific DNA sequence with a kit according to claim 3 .
8. A method for screening seeds for the presence of EE-GH1 comprising detecting an EE-GH1 specific DNA sequence with a kit according to claim 3 .