Method to impregnate a porous bone replacement material
View Patent ↗A method to impregnate a porous bone replacement material ( 1 ) with a liquid impregnating agent ( 5 ), wherein a) the bone replacement material ( 1 ) is or will be enclosed in a chamber ( 2 ) with two openings ( 3, 4 ) that can be closed, b) the impregnating agent ( 5 ) is introduced into the chamber ( 2 ) until the bone replacement material ( 1 ) is at least partly immersed in the impregnating agent ( 5 ), c) one of the two openings ( 3, 4 ) will be closed, d) the chamber ( 2 ) is evacuated at least partly via the other, open, opening ( 4, 3 ), so that the air contained in the pores of the bone replacement material ( 1 ) is at least partly removed from it, e) the vacuum produced in the chamber is terminated again by supplying air or a gas through one of the openings ( 3, 4 ), and f) the vacuum produced in the pores of the bone replacement material ( 1 ) is terminated by the impregnating agent ( 5 ) penetrating into the pores of the bone replacement material ( 1 ) immersed into the impregnating agent ( 5 ).
1. A method for impregnating a porous bone replacement material, comprising:
enclosing a porous bone replacement material in a chamber with two openings that can be closed;
introducing a liquid impregnating agent into the chamber until the porous bone replacement material is at least partly immersed in the liquid impregnating agent;
closing one of the two openings of the chamber;
evacuating the chamber at least partly through the other opening so that the air contained in the pores of the porous bone replacement material is at least partly removed from it; and
terminating the vacuum produced in the chamber by supplying air or a gas through one of the two openings of the chamber so that the vacuum produced in the pores of the porous bone replacement material is terminated by the impregnating agent penetrating into the pores of the porous bone replacement material immersed into the liquid impregnating agent.
2. The method according to claim 1 , wherein the air is removed through the opening provided on the top of the chamber against the gravity vector.
3. The method according to claim 1 , wherein the material is in the form of a dice, cylinder, hollow cylinder, disc, wedge, cone, truncated cone or a sphere.
4. The method according to claim 1 , wherein the liquid impregnating agent comprises one or more osteoinductive and/or osteogenic substances.
5. The method according to claim 4 , wherein the one or more osteoinductive and/or osteogenic substances is a body cell, bone marrow or bone marrow component, blood or blood constituent, or a combination thereof.
6. The method according to claim 1 , wherein the vacuum reduces a pressure initially prevailing in the chamber from 1 bar to below 0.9 bar.
7. The method according to claim 6 , wherein the vacuum reduces the pressure initially prevailing in the chamber to below 0.6 bar.
8. The method according to claim 6 , wherein the vacuum reduces the pressure initially prevailing in the chamber from 1 bar to below 0.2 bar.
9. The method according to claim 6 , wherein the vacuum reduces the pressure initially prevailing in the chamber to below 0.1 bar.
10. The method according to claim 1 , wherein the liquid impregnating agent is introduced into the chamber by aspiration through one of the two openings of the chamber by the flow-through of negative pressure.
11. The method according to claim 1 , wherein the liquid impregnating agent is introduced into the chamber by pressing it through one of the two openings of the chamber by the flow-through of excess pressure.
12. The method according to claim 1 , wherein a pressure in the chamber is cycled more than once.
13. A method for impregnating a porous bone replacement material, comprising:
introducing a non-curing liquid impregnating agent residing in a first chamber into a second chamber that encloses a porous bone replacement material until the porous bone replacement material is at least partly immersed; and
reducing a pressure in the chamber to reduce the amount of air in pores of the bone replacement material and to drive the liquid impregnating agent into the pores.
14. The method of claim 13 , wherein introducing the liquid impregnating agent includes introducing a liquid containing a body cell.
15. The method of claim 13 , wherein introducing the liquid impregnating agent includes introducing a liquid containing a bone marrow component.
16. The method of claim 13 , wherein introducing the liquid impregnating agent includes introducing a liquid containing blood.