Humanized anti-CD4 antibody with immunosuppressive properties
A humanized antibody derived from mouse monoclonal anti-CD4 antibody B-F5 is able to activate CD25+CD4+ regulatory T cells and is useful for preparing immunosuppressive compositions.
1. A humanized anti-CD4 antibody, wherein the humanized anti-CD4 antibody comprises a sequence comprising the complementarity-determining regions (CDRs) of SEQ ID NOs: 10 and 9, displayed in FIGS. 3 and 4 , and wherein the humanized anti-CD4 antibody is capable of activating CD4 + CD25 + regulatory T cells such that the activated CD4 + CD25 + regulatory T cells are able to suppress CD4 + CD45RA+CD25−T cell proliferation in vitro in an allogenic mixed lymphocyte reaction in which the activated CD4 + CD25 + regulatory T cells are cocultured with allogenic CD4 + CD45RA+CD25−T cell in the presence of CD2-depleted peripheral blood mononuclear cells that are syngenic for the CD4 + CD25 + regulatory T cells.
2. A therapeutic composition comprising the humanized antibody according to claim 1 .
3. A method comprising:
preparing a therapeutic composition comprising the humanized anti-CD4 antibody according to claim 1 .