TARGETING VECTOR-PHOSPHOLIPID CONJUGATES
Peptide vectors having high KDR binding affinity and processes for making such vectors are provided. The peptide vectors may be conjugated to phospholipids and included in ultrasound contrast agent compositions. Such ultrasound contrast agents are particularly useful in therapeutic and diagnostic methods, such as in imaging KDR-containing tissue and in the evaluation and treatment of angiogenic processes associated with neoplastic conditions. The present invention also provides processes for the large scale production of highly pure dimeric and monomeric peptide phospholipid conjugates as well as precursor materials used to form the conjugates. The present invention further provides processes for the large scale production of highly pure peptide phospholipid conjugates which contain very low levels of TFA.
1 - 39 . (canceled)
40 . A method of making a peptide phospholipid conjugate comprising, conjugating a peptide selected form the group consisting of SEQ ID NO. 2, Ac-AGPTWCEDDWYYCWLFGTGGGK[K(ivDde)]-NH 2 cyclic (6-13) disulfide, Ac-VCWEDSWGGEVCFRYDPGGGK(Adoa-Adoa)-NH 2 cyclic (2-12) disulfide, SEQ ID NO. 5 and Ac-AGPTWCEDDWYYCWLFGTGGGK[Ac-VCWEDSWGGEVCFRYDPGGGK(-Adoa-Adoa-Glut-K)-NH 2 cyclic (2-12) disulfide]-NH 2 cyclic (6-13) disulfide with a phospholipid.
41 . The method of claim 40 , wherein the phospholipid is pegylated.
42 . The method of claim 41 , wherein the pegylated phospholipid is DSPE-PEG2000-NH 2 .
43 . The method of claim 42 , wherein the conjugation comprises reacting a mono NHS ester of the peptide with a free amino group of the phospholipid salt.
44 . A method of making a peptide phospholipid conjugate having low levels of TFA comprising the steps of:
converting peptide dimer TFA salts to an acetate salt via anion exchange; and
conjugating the peptide dimer to a phospholipid.
45 . The method of claim 44 , wherein the peptide comprises a peptide dimer.
46 . The method of claim 45 , wherein the peptide dimer comprises Ac-AGPTWCEDDWYYCWLFGTGGGK[Ac-VCWEDSWGGEVCFRYDPGGGK(-Adoa-Adoa-Glut-K)-NH 2 cyclic (2-12) disulfide]-NH 2 cyclic (6-13) disulfide.
47 . The method of claim 44 , wherein the anion exchange chromatography comprises a step gradient of ammonium acetate.
48 . The method of claim 44 , wherein the phospholipid is pegylated.
49 . The method of claim 48 , wherein the pegylated phospholipid is DSPE-PEG2000-NH 2 .
50 . A method of making a peptide-phospholipid conjugate having low levels of TFA comprising eluting a peptide phospholipid conjugate and TFA ions in a size exclusion column.
51 . The method of claim 50 , wherein said eluting is in the presence of ammonium bicarbonate.
52 . The method of claim 50 , wherein the peptide phospholipid conjugate comprises a pegylated phospholipid.
53 . The method of claim 52 , wherein the pegylated phospholipid is DSPE-PEG2000-NH 2 .
54 . The method of claim 50 , wherein the peptide-phospholipid conjugate comprises a peptide dimer.
55 . The method of claim 54 , wherein the peptide dimer comprises Ac-AGPTWCEDDWYYCWLFGTGGGK[Ac-VCWEDSWGGEVCFRYDPGGGK(-Adoa-Adoa-Glut-K)-NH 2 cyclic (2-12) disulfide]-NH 2 cyclic (6-13) disulfide.
56 . The method of claim 50 , wherein the peptide-phospholipid conjugate comprises a peptide monomer.
57 . The method of claim 56 , wherein the monomer is selected from the group consisting of: SEQ ID NO.2, Ac-AGPTWCEDDWYYCWLFGTGGGK[K(ivDde)]-NH 2 cyclic (6-13) disulfide, Ac-VCWEDSWGGEVCFRYDPGGGK(Adoa-Adoa)-NH 2 cyclic (2-12) disulfide, and SEQ ID NO. 5.