Methods of inhibiting T1R1/T1R3 heteromeric umami taste receptors
View Patent ↗Methods of inhibiting umami taste receptors are provided. These methods comprise contacting T1R1/T1R3 umami taste receptors with a sweet-taste inhibitor that also inhibits both the T1R1/T1R3 umami taste receptor and the T1R2/T1R3 sweet taste receptor.
1. A method of inhibiting the T1R1/T1R3 umami taste receptor comprising contacting said receptor with a sweet-taste inhibitor compound that specifically interacts with the T1R3 subunit comprised in the T1R1/T1R3 umami taste receptor and the T1R2/T1R3 sweet taste receptor, wherein said compound inhibits both the T1R1/T1R3 umami taste receptor and the T1R2/T1R3 sweet taste receptor.
2. The method of claim 1 , wherein said inhibitor is lactisole.
3. The method of claim 1 , wherein said compound is identified using a T1R3 expressing cell.
4. The method of claim 3 , wherein said method uses a cell line that co-expresses T1R1 and T1R3.
5. The method of claim 4 , wherein said cell line is a HEK-Gα 15 cell line.
6. The method of claim 4 , wherein said cell line stably expresses said receptors.
7. The method of claim 4 , wherein said cell line transiently expresses said receptors.
8. The method of claim 3 , wherein said method uses a cell line that co-expresses T1R2 and T1R3.
9. The method of claim 1 wherein the effect of said compound on sweet or umami taste is determined in a taste test.
10. The method of claim 1 wherein the T1R1/T1R3 umami taste receptor is further contacted with a second compound to determine if said second compound affects the specific interaction of said sweet taste inhibitor compound with said T1R3 subunit in said T1R1/T1R3 receptor.
11. The method of claim 10 wherein the effect of said second compound is determined in a taste test.