IP Library › Granted Patent US 8,039,590
Granted Patent B2
US 8,039,590 · App. 12/270,857 · Granted Oct 18, 2011

Heteromeric taste receptors comprising T1R2/T1R3 domains

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Quick Facts
Patent No.
US 8,039,590
App. No.
12/270,857
Granted
Oct 18, 2011
Kind
B2
Abstract

Heteromeric taste receptors are provided. These receptors comprise a first polypeptide which comprises extracellular and transmembrane domains wherein the transmembrane domains are at least 95% identical to the transmembrane domains of specific T1R2 polypeptides, and the extracellular domains are at least 95% identical to the corresponding extracellular domains of the specific T1R2 polypeptide or a different GPCR; and a second polypeptide which comprises extracellular and transmembrane domains wherein the transmembrane domains are at least 95% identical to the transmembrane domains of specific T1R3 polypeptides, and the extracellular domains are at least 95% identical to the corresponding extracellular domains of the specific T1R3 polypeptide or that of a different GPCR.

Claims (17)

1. A heteromeric taste receptor comprising:

(a) a first polypeptide comprised of extracellular and transmembrane domains wherein said transmembrane domains are at least 95% identical to the transmembrane domains of the T1R2 polypeptide of SEQ ID NO: 6, and the extracellular domains are at least 95% identical to the corresponding extracellular domains of said T1R2 polypeptide and;

(b) a second polypeptide comprised of extracellular and transmembrane domains wherein said transmembrane domains are at least 95% identical to the transmembrane domains of the T1R3 polypeptide of SEQ ID NO: 7, and the extracellular domains are at least 95% identical to the corresponding extracellular domains of said T1R3 polypeptide.

2. The heteromeric taste receptor of claim 1 , wherein the transmembrane domains of the first polypeptide are at least 96% identical to the transmembrane domains of the T1R2 polypeptide of SEQ ID NO: 6.

3. The heteromeric taste receptor of claim 1 , wherein the transmembrane domains of the first polypeptide are at least 97% identical to the transmembrane domains of the T1R2 polypeptide of SEQ ID NO: 6.

4. The heteromeric taste receptor of claim 1 , wherein the transmembrane domains of the first polypeptide are at least 98% identical to the transmembrane domains of the T1R2 polypeptide of SEQ ID NO: 6.

5. The heteromeric taste receptor of claim 1 , wherein the transmembrane domains of the first polypeptide are at least 99% identical to the transmembrane domains of the T1R2 polypeptide of SEQ ID NO: 6.

6. The heteromeric taste receptor of claim 1 , wherein the transmembrane domains of the first polypeptide are identical to those of the T1R2 polypeptide of SEQ ID NO: 6.

7. The heteromeric taste receptor of claim 1 , wherein the transmembrane domains of the first polypeptide are at least 96% identical to the transmembrane domains of the T1R3 polypeptide of SEQ ID NO: 7.

8. The heteromeric taste receptor of claim 1 , wherein the transmembrane domains of the second polypeptide are at least 97% identical to the transmembrane domains of the T1R3 polypeptide of SEQ ID NO: 7.

9. The heteromeric taste receptor of claim 1 , wherein the transmembrane domains of the second polypeptide are at least 98% identical to the transmembrane domains of the T1R3 polypeptide of SEQ ID NO: 7.

10. The heteromeric taste receptor of claim 1 , wherein the transmembrane domains of the second polypeptide is at least 99% identical to the transmembrane domains of the T1R3 polypeptide of SEQ ID NO: 7.

11. The heteromeric taste receptor of claim 1 , wherein the transmembrane domains of the second polypeptide are identical to that of the T1R3 polypeptide of SEQ ID NO: 7.

12. The heteromeric taste receptor of claim 1 , wherein said heteromeric taste receptor is expressed in a eukaryotic cell.

13. The heteromeric taste receptor of claim 1 , wherein said heteromeric taste receptor is expressed in an HEK-293 cell.

14. The heteromeric taste receptor of claim 12 , wherein the cell contains a G protein.

15. The heteromeric taste receptor of claim 14 , wherein the G protein is Gα 15 or Gα 16 .