Microfluidic device
View Patent ↗A method of controlling the flow of an aqueous fluid in a microfluidic device, which makes use of a fluid gate comprising a UCST (upper critical solubility temperature) polymer and which allows fluid to flow by raising the temperature of the fluid gate above the USCT. Also provided is a device incorporating such a fluid gate and the use of such a device to detect an analyte in an aqueous fluid sample.
1. A method of controlling the flow of an aqueous fluid in a microfluidic device, which method comprises the steps of:
(a) adding a fluid sample to a microfluidic device, which comprises an inlet region and a downstream detection zone which are connected by one or more channels, wherein at least one channel contains a fluid gate comprising a UCST (upper critical solubility temperature) polymer, wherein said UCST polymer is derived from the polymerization of a MEDSAH ([2-(methacryloyloxy)ethyl]-dimethyl-(3-sulfopropyl)ammonium hydroxide) monomer;
(b) allowing the sample to be retained within the device for a period of time by the fluid gate by maintaining the temperature of the fluid gate below the USCT of the polymer such that the fluid gate is closed, followed by
(c) applying heat to raise the temperature of the fluid gate above the USCT of the polymer such that the fluid gate opens and the fluid sample is able to flow across the fluid gate to a region downstream from said gate, wherein the cross-sectional area of the polymer is 50% or less of the channel cross-sectional area.
2. A method according to claim 1 wherein the fluid gate is provided upstream or downstream of the detection zone.
3. A method according to claim 1 which further comprises the step of detecting an analyte in the fluid sample.
4. A method according to claim 1 wherein the fluid sample is a liquid sample.
5. A method according to claim 1 wherein the UCST polymer is in the form of a polymer brush.
6. A method according to claim 5 wherein the polymer brush has a thickness of greater than 50 nm.
7. A method according to claim 1 wherein the UCST polymer is a zwitterionic polymer.
8. A method according to claim 1 wherein the UCST polymer comprises sulphobetaine polymers.
9. A method according to claim 1 wherein the cross-sectional area of the polymer is 25% or less of the channel cross-sectional area.