DNA conformation (loop structures) in normal and abnormal gene expression
View Patent ↗Method of detection or diagnosis of abnormal gene expression in an individual comprising determining in a sample from the individual the presence or absence of a chromosome structure in which two separate regions of the gene have been brought into close proximity, to thereby detect or diagnose whether the individual has abnormal gene expression.
1. A method of making a predefined ligated DNA from a DNA sample from a human individual, comprising the steps of:
(a) identifying two Checkpoint Charlie (CC) markers in a human gene using the algorithm of Table 1, wherein each CC marker has a reverse algorithmic score of more than 0.9 according to the algorithm defined in Table 1 and wherein the two CC markers together form a juxtaposition in said gene;
(b) cross-linking the identified CC markers in the DNA sample;
(c) digesting the cross-linked DNA with at least one enzyme;
(d) ligating the digested DNA to create a new predefined ligated DNA sequence not previously present in the sample;
(e) providing PCR primers capable of amplifying the ligated DNA in a PCR reaction; and
(f) amplifying the ligated DNA in a PCR reaction using said PCR primers.
2. A method according to claim 1 wherein one or both CC markers have a length of 5 to 20 bases.