Method for cloning and expression of StuI restriction endonuclease and StuI methylase in
View Patent ↗The present invention relates to compositions including: (1) isolated DNA encoding the StuI restriction endonuclease and isolated DNA encoding cognate and non-cognate methylase; (2) vectors and cells containing the isolated DNA; and (3) methods for producing the StuI restriction endonuclease.
1. An isolated DNA encoding a restriction endonuclease comprising SEQ ID NO: 2.
2. A recombinant vector comprising the isolated DNA according to claim 1 .
3. A host cell transformed by a recombinant vector according to claim 2 .
4. A method of producing an endonuclease, comprising: culturing a host cell transformed with a vector according to claim 2 under conditions suitable for expression of the endonuclease.
5. The isolated DNA of claim 1 , wherein the restriction endonuclease cleaves DNA at the nucleotide sequence AGGCCT.
6. An isolated nucleic acid that encodes a polypeptide with at least 90% sequence identity to SEQ ID NO: 2.
7. A recombinant vector comprising the isolated nucleic acid according to claim 6 .
8. A host cell transformed by a recombinant vector according to claim 7 .