Intelligent system and method for automated packing of chromatography columns
View Patent ↗The present invention provides an automated system and method for packing chromatography columns. In one embodiment, the system first determines the type of media that is present in the column, and uses this information to generate an automated procedure to pack the specific media type. In a second embodiment, the media type is made known to the system, such as via input from the operator. The system then uses this information, in the same manner as in the first embodiment, to generate an automated procedure to pack the specific media type. Finally, in a third embodiment, parameters such as column height and rate of compression are made known to the system, such as via input from the operator. The system then packs the column in accordance with these supplied parameters.
1. A method of packing a media bed of a chromatography column through which a fluid is flowed, said column having an adjuster for compressing said bed, wherein said adjuster is subjected to a compression force from said media and a hydraulic force from said fluid, and means for determining at least two of said compression force, said hydraulic force and the total force to which said adjuster is subjected, said method comprising:
actuating said adjuster to compress said bed;
determining at least one of said forces;
calculating a value based on said determined forces; and
ceasing movement of said actuator when said calculated value reaches a predetermined level.
2. The method of claim 1 , wherein said means determines said total force, and said calculated value is equal to said total force.
3. The method of claim 1 , wherein said means determines said hydraulic force and said total force, and said calculated value is the ratio of said hydraulic force to said total force.
4. The method of claim 1 , wherein said means determines said compression force, and said calculated value is said compression force.
5. The method of claim 1 , wherein said means determines said compression force and said total force, and said calculated value is the ratio of said compression force to said total force.
6. A method of packing a media bed of a chromatography column through which a fluid is flowed, said column having an adjuster for compressing said bed, wherein said adjuster is subjected to a compression force from said media and a hydraulic force from said fluid, and means for determining at least one of said compression force, said hydraulic force and the total force to which said adjuster is subjected, said
method comprising:
actuating said adjuster to compress said bed;
determining at least one of said forces;
calculating a time derivative of said determined forces; and
ceasing movement of said actuator when said calculated time derivative reaches a predetermined level.
7. The method of claim 6 , wherein said means determines said total force.
8. The method of claim 6 , wherein said means determines said compression force.