Non-desensitizing mutant of the transient receptor potential TRPM5 ion channel
View Patent ↗There exists a need in the art for high throughput screening assays that can identify compounds that specifically modulate the activity of fast-acting ion channels, such as TRPM5. Current methods, especially electrophysiological, suffer from a lack of sensitivity, rapid signal loss, low throughput, and are labor intensive. The claimed methods and compositions provide electrophysiology methods that allow prolonged sample testing and fluorescent assays with an optical readout that gives rapid readout of the results, has a high signal to noise background ratio, are easy to use, can be modified for automation and miniaturization, and provide verification that a compound specifically modulates TRPM5.
1. An isolated polynucleotide, wherein the polynucleotide has the sequence of SEQ ID NO: 1.
2. An isolated polynucleotide, wherein the polynucleotide encodes a non-desensitizing TRPM5 ion channel having the sequence of SEQ ID NO: 2.
3. A recombinant vector comprising the isolated polynucleotide of claim 1 .
4. A recombinant vector comprising the isolated polynucleotide of claim 2 .
5. An isolated host cell comprising the vector of claim 3 .
6. A method of making an isolated polypeptide comprising: (a) culturing the host cell of claim 5 under conditions such that said polypeptide is expressed; and (b) recovering said polypeptide.
7. An isolated host cell comprising the vector of claim 4 .
8. A method of making an isolated polypeptide comprising: (a) culturing the host cell of claim 7 under conditions such that said polypeptide is expressed; and (b) recovering said polypeptide.