Histological facilitation systems and methods
Systems, methods, and other modalities are described for generating or otherwise handling images or other data indicating (a) an extraction of chemically treated tissue frozen in vivo, (b) a treatment of a tissue sample in a chamber extended into tissue of an organism, and/or (c) cells to which an optical enhancement material was applied in vivo. Several contexts in which such indications facilitate histological evaluation are likewise described.
1 . A medical or veterinary system comprising:
first means for applying a treatment material to tissue of an organism in vivo; and
second means for freezing and removing at least a portion of the tissue from the organism.
2 . A medical or veterinary system comprising:
a first dispenser configured to apply a first treatment material to tissue of an organism in vivo;
a cooling component configured to freeze at least some of the tissue in vivo; and
an extraction element configured to remove at least a portion of the tissue from the organism.
3 . The medical or veterinary system of claim 2 , further comprising:
circuitry for actuating the first dispenser in response to contemporaneous user input.
4 . The medical or veterinary system of claim 2 , further comprising:
circuitry for positioning at least a portion of the first dispenser.
5 . The medical or veterinary system of claim 2 , further comprising:
circuitry for processing data from one or more assay protocols performed upon the portion of the tissue.
6 . The medical or veterinary system of claim 2 , further comprising:
circuitry for processing data from one or more biomarker detection protocols performed upon (at least some of) the portion of the tissue.
7 . The medical or veterinary system of claim 2 , further comprising:
at least one medium bearing an operational setting value usable in laser scanning equipment for analyzing the portion of the tissue.
8 . The medical or veterinary system of claim 2 , further comprising:
at least one medium bearing an operational setting value usable in a mass spectroscope for analyzing the portion of the tissue.
9 . The medical or veterinary system of claim 2 , further comprising:
at least one medium bearing an operational setting value usable in a microscope for analyzing the portion of the tissue.
10 . The medical or veterinary system of claim 2 , further comprising:
laser scanning equipment operable for receiving at least some of the extraction element of the device.
11 . The medical or veterinary system of claim 2 , further comprising:
imaging equipment operable for receiving at least some of the extraction element of the device.
12 . The medical or veterinary system of claim 2 in which the extraction element comprises:
a recessed portion configured to contain the portion of the tissue.
13 . The medical or veterinary system of claim 2 , further comprising:
one or more conduits operably coupling imaging equipment with a portion of the extraction element configured to support the portion of the tissue.
14 - 15 . (canceled)
16 . The medical or veterinary system of claim 2 in which the first treatment material comprises:
a permeabilizing agent.
17 . The medical or veterinary system of claim 2 , further comprising:
a microinjection module configured to penetrate at least one cell of the tissue in vivo.
18 . The medical or veterinary system of claim 2 , further comprising:
an electropermeabilization module configured to permeabilize at least one cell of the tissue in vivo.
19 . The medical or veterinary system of claim 2 , further comprising:
circuitry for generating one or more quantifications from an optical field of the tissue from the organism.
20 . The medical or veterinary system of claim 2 , in which the extraction element comprises:
a microtome configured to section the portion of the tissue.
21 . The medical or veterinary system of claim 2 , in which the extraction element comprises:
a device configured to extract the portion of the tissue by dividing a sample of the tissue.
22 . The medical or veterinary system of claim 2 , in which the extraction element comprises:
a component configured to observe the portion of the tissue in a first cavity and to transmit an output via one or more conduits.
23 . (canceled)
24 . The medical or veterinary system of claim 2 , further comprising:
circuitry for observing the tissue configured to cause a presentation of an output on one or more physical media.
25 . The medical or veterinary system of claim 2 , further comprising:
circuitry for transmitting a go/no-go result of the first treatment material on one or more physical media.
26 . The medical or veterinary system of claim 2 , further comprising:
an electron microscope configured to observe the portion of the tissue and :to provide an output on one or more physical media.
27 . The medical or veterinary system of claim 2 , further comprising:
a fluorescence microscope configured to observe the portion of the tissue and to provide an output on one or more physical media.
28 . The medical or veterinary system of claim 2 , further comprising:
a confocal microscope configured to observe the portion of the tissue and to provide an output on one or more physical media.
29 . The medical or veterinary system of claim 2 , further comprising:
a spectrometer configured to observe the portion of the tissue and to provide an output on one or more physical media.
30 . The medical or veterinary system of claim 2 , further comprising:
an imaging system configured to observe the portion of the tissue and to provide an output on one or more physical media.
31 . The medical or veterinary system of claim 2 , further comprising:
a nuclear magnetic resonance imaging system configured to observe the portion of the tissue and to provide an output on one or more physical media.
32 . The medical or veterinary system of claim 2 , further comprising:
circuitry for transmitting energy into the portion of the tissue; and
circuitry for capturing an image of the portion of the tissue.
33 . The medical or veterinary system of claim 2 , further comprising:
one or more handling control surfaces configured to permit a user to extend an entirety of the extraction element into the organism.
34 . The medical or veterinary system of claim 2 , in which the extraction element comprises:
a first separable module containing a cavity and supportable by and separable from a remainder of the extraction element.
35 . The medical or veterinary system of claim 2 , further comprising:
one or more media bearing an attribute of a macromolecule relating to the tissue.
36 . The medical or veterinary system of claim 2 , further comprising:
one or more media bearing a morphological category relating to a portion of the tissue of the organism.
37 . The medical or veterinary system of claim 2 , further comprising:
circuitry for selecting a stain effective for indicating whether the tissue includes a chromosomal abnormality.
38 . The medical or veterinary system of claim 2 , further comprising:
circuitry for causing the portion of the tissue to come into contact with a stain effective for indicating whether the tissue apparently exhibits an attribute of interest.
39 . The medical or veterinary system of claim 2 in which the first dispenser comprises:
a permeabilizing agent of the first treatment material.
40 . The medical or veterinary system of claim 2 in which the first dispenser comprises:
a luminescent marking agent of the first treatment material.
41 . The medical or veterinary system of claim 2 in which the first dispenser comprises:
a stain of the first treatment material.
42 . The medical or veterinary system of claim 2 , further comprising:
circuitry for configuring a result of the first treatment material to include one or more size-descriptive quantities relating to the tissue.
43 . The medical or veterinary system of claim 2 , further comprising:
circuitry for generating one or more size-descriptive quantities relating to the portion of the tissue from the organism.