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View Patent ↗The present invention relates to plant biotechnology and specifically to a method for genetically transforming Camelina sativa with Agrobacterium -mediated transformation system. It comprises Camelina sativa for producing homologous and heterologous recombinant products including oil and protein products and assessing and screening the efficacy of plant transformation. Also disclosed are transgenic Camelina sativa plants, seeds as well as cells, cell-lines and tissue of Camelina sativa.
1. A method for genetically transforming a Camelina sativa plant comprising the steps of:
a) collecting Camelina sativa seeds from a plant grown in green house or growth chamber,
b) sterilizing the seeds and growing seedlings in vitro;
c) providing explants from in vitro grown Camelina sativa leaves;
d) inoculating the explants with Agrobacterium strain containing at least one recombinant DNA construct and optionally a selection marker;
e) allowing transformation to take place on culture medium supplemented with 2% sucrose;
f) inducing formation of one or more shoots and roots from transformed explants on a cell culture medium, said medium containing 2% sucrose;
g) allowing elongation of transgenic shoots and roots on a medium containing 2-3% sucrose; and
h) growing the shoots into transgenic Camelina sativa plants.
2. The method of claim 1 , wherein Agrobacterium strain of step d is free from selection markers.
3. The method of claim 1 , wherein the recombinant DNA construct contains a sequence encoding a homologous or heterologous gene product.
4. The method of claim 2 , wherein the recombinant DNA construct contains a sequence encoding a homologous or heterologous gene product.
5. The method of claim 1 , wherein the transgenic Camelina sativa cell or cell line is produced.
6. The method of claim 2 , wherein the transgenic Camelina sativa cell or cell line is produced.
7. The method of claim 1 , wherein the transgenic Camelina sativa seed is produced.
8. The method of claim 7 , wherein the seed contains heterologous or homolgous recombinant products.
9. The method of claim 2 , wherein the transgenic Camelina sativa seed is produced.
10. The method of claim 9 , wherein the seed contains heterologous or homologous recombinant products.