Composition and method for the release of protected peptides from a resin
View Patent ↗The present invention provides a composition and a method for cleaving a peptide from a solid support resin. Hydrochloric acid in an organic water miscible solvent is used to cleave the peptide-resin attachment. Optionally, trifluoroethanol or hexafluoroisopropanol may be added to the cleavage composition to improve results. When using the present cleavage composition, an evaporation or other step to remove carboxylic byproducts is not necessary following the cleavage reaction. After the resin is filtered out of the cleavage mixture, the peptide may be immediately precipitated with water.
1. A method of peptide synthesis comprising:
a. cleaving a resin bound intermediate peptide fragment from a resin by subjecting the resin-peptide attachment to a composition comprising:
an organic water miscible solvent,
from 0.05 N to 0.5 N hydrochloric acid, and
from about 1% to about 12% of a fluorinated alcohol, thus creating a cleavage mixture comprising a non-resin bound intermediate peptide fragment;
b. precipitating said intermediate peptide fragment by treating said cleavage mixture with water; and
c. combining at least two intermediate peptide fragments generated from steps a and b and performing a fragment condensation reaction with said at least two intermediate peptide fragments;
wherein said method does not include an evaporation step in between steps a-c, and wherein said cleaving step a produces substantially no carboxylic acid byproducts.
2. The method of claim 1 , wherein the concentration of hydrochloric acid is 0.1 N.
3. The method of claim 1 , wherein the fluorinated alcohol is trifluoroethanol or hexafluoroisopropanol.
4. The method of claim 3 , wherein the composition comprises from 5% to 10% trifluoroethanol.
5. The method of claim 3 , wherein the composition comprises about 10% trifluoroethanol.
6. The method of claim 3 , wherein the composition comprises from about 5% to about 10% hexafluoroisopropanol.
7. The method of claim 3 , wherein the composition comprises about 10% hexafluoroisopropanol.
8. The method of claim 3 , wherein the organic water miscible solvent is dimethylformamide, N-methylpyrrolidone, dimethylacetamide, dimethyl sulfoxide, tetrahydrofuran, or dioxane.
9. The method of claim 8 , wherein the organic water miscible solvent is dimethylformamide.
10. The method of claim 3 , wherein during cleaving the resin-peptide is subjected to about 2 mL to about 22 mL of composition per gram resin-peptide.
11. The method of claim 3 , wherein during cleaving the resin-peptide is subjected to about 4 mL to about 10 mL of composition per gram resin-peptide.
12. The method of claim 3 , wherein during cleaving the resin-peptide is subjected to about 4 mL to about 6 mL of composition per gram resin-peptide.
13. The method of claim 3 , wherein during precipitating the intermediate peptide fragment, the cleavage mixture is treated with at least about 4 mL of water per gram of resin-peptide.