Method for delivery across the blood brain barrier
The present invention provides compositions and methods useful for delivering agents to target cells or tissues, for example nerve cells and other cells in the central nervous system. The compositions and methods are useful for delivering agents across the blood-brain barrier. The present invention also provides methods of using the compositions provided by the present invention to deliver agents, for example therapeutic agents for the treatment of neurologically related disorders.
1. A composition for targeted delivery of an effector agent to a target cell, wherein the composition comprises;
at least one targeting agent, wherein the targeting agent is a peptide of the rabies virus glycoprotein (RVG) which can bind to an acetylcholine receptor on the target cell, wherein the peptide is less than 50 amino acids and comprises SEQ ID NO:13 or a fragment of at least 15 contiguous amino acids of SEQ ID NO: 13 which can bind to an acetylcholine receptor on the target cell,
at least one carrier particle attached to the targeting agent, and
at least one effector agent, wherein the effector agent is indirectly or directly linked with the carrier particle or encapsulated within the carrier particle.
2. The composition of claim 1 , wherein the carrier particle comprises a cell permeable agent.
3. The composition of claim 1 , wherein the carrier particle is a liposome, polyarginine, protamine, or cyclodextrin-based nanoparticle.
4. The composition of claim 1 , wherein the carrier particle is a cell permeable peptide.
5. The composition of claim 4 , wherein the cell permeable peptide is a polymeric arginine residue of varying lengths.
6. The composition of claim 5 , wherein the polymeric arginine residue is 11 residues, 9 residues, 7 residues, or 5 residues.
7. The composition of claim 1 , wherein the target cell is located inside the blood brain barrier.
8. The composition of claim 1 , wherein the target cell is selected from a neuron, neuronal cell, brain cells, glial, astrocyte or a neuronal supporting cell.
9. The composition of claim 1 , wherein the target cell comprises the acetyl choline receptor (AchR).
10. The composition of claim 9 , wherein the acetyl choline receptor comprises the α subunit of AchR.
11. The composition of claim 10 , wherein the α subunit of AchR is α1 or α7 subunit of AchR.
12. The composition of claim 1 , wherein the effector agent is a nucleic acid.
13. The composition of claim 12 , wherein the nucleic acid is selected from the group consisting of: RNA, siRNA, mRNA, tRNA, miRNA, shRNA or combinations thereof.
14. The composition of claim 12 , wherein the nucleic acid is selected from the group consisting of: DNA, antisense nucleic acids, oligonucleic acids, peptide nucleic acid (PNA), pseudo-complementary PNA (pcPNA), locked nucleic acid (LNA), and antigomirs.
15. The composition of claim 1 , wherein the effector agent is a small molecule.
16. The composition of claim 1 , wherein the effector agent is selected from the group consisting of: a protein, peptide, peptidomimetic, aptamer, avimer or antibody.
17. The composition of claim 1 , further comprising a blood brain barrier targeting agent.
18. The composition of claim 1 , wherein the composition further comprises a pharmaceutically acceptable carrier.
19. The composition of claim 1 , wherein the composition is administrable by subcutaneous, intravenous, intracranial administration or oral injection.
20. The composition of claim 1 , wherein the target cell is across the blood brain barrier or within the central nervous system.