Separating material and method for collecting cell or the like using the same
View Patent ↗The present invention provides a novel material useful for selectively isolating a cell such as monocyte and the like or a protein from a body fluid and a production method thereof, a physiological material using the material and an isolation material using the physiological material, as well as a method of harvesting a cell such as monocyte and the like using the isolation material, a method of harvesting a protein and a method of preparing a dendritic cell.
1. An oligosaccharide-containing polyurethane represented by the following formula [1-1]:
wherein
R 1 is a divalent C 1-16 hydrocarbon group optionally having substituent(s),
R 2 is a divalent group containing the same or different 1 to 100 units in total selected from a C 2-12 oxyalkylene unit and a divalent C 2-12 hydrocarbon unit optionally having substituent(s),
R 3 is a C 2-4 alkylene group,
R 4 is a monosaccharide- or disaccharide-derived sugar residue,
OLS is an oligosaccharide skeleton having two primary hydroxyl groups,
r shows the total number of secondary hydroxyl groups of oligosaccharide,
p and q are each an integer within the range of 1≦p≦r, 0≦q≦r−1, and
m and n are each a repeat unit number, m is an integer of 0-1000 and n is an integer of 1-1000, and n/(m+n) is a number within the range of 0.01-1.00,
the following formula [1-2]:
wherein
R 1 is a divalent C 1-16 hydrocarbon group optionally having substituent(s),
R 2 is a divalent group containing the same or different 1 to 100 units in total selected from a C 2-12 oxyalkylene unit and a divalent C 2-12 hydrocarbon unit optionally having substituent(s),
R 3 is a C 2-4 alkylene group,
OLS is an oligosaccharide skeleton having two primary hydroxyl groups,
r shows the total number of secondary hydroxyl groups of oligosaccharide,
p and q are each an integer within the range of 1≦p≦r, 0≦q≦r−1, and
m and n are each a repeat unit number, m is an integer of 0-1000 and n is an integer of 1-1000, and n/(m+n) is a number within the range of 0.01-1.00,
the following formula [2-1]:
wherein
R 1 is a divalent C 1-16 hydrocarbon group optionally having substituent(s),
R 2 is a divalent group containing the same or different 1 to 100 units in total selected from a C 2-12 oxyalkylene unit and a divalent C 2-12 hydrocarbon unit optionally having substituent(s),
OLS is an oligosaccharide skeleton having two primary hydroxyl groups,
r shows the total number of secondary hydroxyl groups of oligosaccharide,
XM is an acid-containing group wherein M is a hydrogen atom, an alkali metal atom, an ammonium group or an organic amino group, and X is an acid-derived moiety,
s shows the number of the secondary hydroxyl groups of an oligosaccharide modified with an acid-containing group, which is an integer within the range of 1 ≦s≦r, and
m and n are each a repeat unit number, m is an integer of 0-1000 and n is an integer of 1-1000, and n/(m+n) is a number within the range of 0.01-1.00, or
the following formula [3-1]:
wherein
R 1 is a divalent C 1-16 hydrocarbon optionally having substituent(s),
R 2 is a divalent group containing the same or different 1 to 100 units in total selected from a C 2-12 oxyalkylene unit and a divalent C 2-12 hydrocarbon unit optionally having substituent(s),
R 3 is a C 2-4 alkylene group,
R 4 is a monosaccharide- or disaccharide-derived sugar residue,
XM is an acid-containing group wherein M is a hydrogen atom, an alkali metal atom, an ammonium group or an organic amino group, and X is an acid-derived moiety, s shows the number of hydroxyl groups of a sugar residue R 4 modified with an acid-containing group, which is an integer within the range of 1≦s≦4,
OLS is an oligosaccharide skeleton having two primary hydroxyl groups,
r shows the total number of secondary hydroxyl groups of oligosaccharide,
p and q are each an integer within the range of 1≦p≦r, 0≦q≦r−1, and
m and n are each a repeat unit number, m is an integer of 0-1000 and n is an integer of 1-1000, and n/(m+n) is a number within the range of 0.01-1.00.
2. A physiological material comprising the polyurethane of claim 1 .
3. An isolation material comprising the polyurethane of claim 1 at least on a part of a surface thereof.
4. The isolation material of claim 3 , which is a cell isolation material, a protein isolation material or a blood isolation material.
5. The isolation material of claim 3 , having the form of a filter.
6. An isolation device comprising a container provided with a liquid inlet and/or a liquid outlet, wherein the container comprises the isolation material of claim 3 in at least one part thereof.
7. The isolation device of claim 6 , which is a cell isolation device or a protein isolation device.
8. A method of harvesting a cell comprising performing
1) a step for trapping a cell on an isolation material by contacting a body fluid with the isolation material comprising, on at least a part of a surface thereof, a substance having a sugar structure,
2) a step for removing untrapped cells by washing the isolation material with a washing liquid, and
3) a step for collecting a trapped cell from the isolation material, in this order, wherein the substance having a sugar structure is the polyurethane of claim 1 .