Circulating tumor cell assay
Methods for the detection, enumeration and analysis of circulating tumor cells expressing insulin-like growth factor-1 receptors (IGF-1R) are disclosed. These methods are useful for cancer screening and staging, development of treatment regimens, and for monitoring for treatment responses, cancer recurrence or the like. Test kits that facilitate the detection, enumeration and analysis of such circulating tumor cells are also provided.
1. A method for predicting efficacy of IGF-1R antagonist therapy in a patient, comprising the steps of:
a) preparing a sample wherein a blood sample from the patient is mixed with an antibody to epithelial cell adhesion molecule (EpCAM), so as to obtain a cell population enriched for tumor cells;
b) contacting the enriched cell population with an anti-cytokeratin antibody labeled with fluorescein isothiocyanate (FITC);
c) contacting the enriched cell population with a phycoerythrin (PE)-labeled antibody to insulin-like growth factor receptors (IGF-1R); and
d) determining the presence of cells bound by both the anti-cytokeratin antibody and the anti-IGF-1R antibody;
wherein the presence of cells bound by both antibodies is predictive of efficacy of IGF-1R antagonist therapy in the patient.
2. A method for monitoring efficacy of an IGF-1R antagonist therapy-in a patient, comprising the steps of:
a) preparing a first sample wherein a first blood sample from the patient is mixed with an antibody to epithelial cell adhesion molecule (EpCAM), so as to obtain a cell population enriched for tumor cells;
b) contacting the enriched cell population with an anti-cytokeratin antibody labeled with fluorescein isothiocyanate (FITC);
c) contacting the enriched cell population with a phycoerythrin (PE)-labeled antibody to insulin-like growth factor receptors (IGF-1R);
d) determining the presence and number of cells bound by both the anti-cytokeratin antibody and the anti-IGF-1R antibody;
e) preparing a second sample from a second blood sample from the patient, after administration of an IGF-1R antagonist therapy, wherein the second blood sample is mixed with the antibody to EpCAM, and performing steps b)-d) on the second sample; and
f) comparing the number of cells bound by both the anti-cytokeratin antibody and the anti-IGF-1R antibody in the first sample to the number of cells bound by both antibodies in the second sample;
wherein a lower number of cells bound by both antibodies in the second sample is indicative of efficacy of the IGF-1R antagonist therapy in the patient.