Generation of plants with improved pathogen resistance
View Patent ↗The present disclosure is directed to plants that display a modified pathogen resistance phenotype (e.g., increased nematode resistance) due to altered expression of an NMR nucleic acid. The invention is further directed to methods of generating plants with a modified pathogen resistance phenotype.
1. A transgenic plant having stably incorporated into its genome a DNA construct comprising a nucleotide sequence that encodes a protein having nematode resistance activity, wherein said nucleotide sequence is selected from the group consisting of:
a) the nucleotide sequence set forth as SEQ ID NO: 61;
b) a nucleotide sequence having at least 90% sequence identity to the nucleotide sequence set forth as SEQ ID NO: 61;
c) a nucleotide sequence encoding a polypeptide comprising the amino acid sequence set forth as SEQ ID NO: 62; and,
d) a nucleotide sequence encoding a polypeptide having at least 90% sequence identity to the amino acid sequence set forth as SEQ ID NO: 62;
wherein the nucleotide sequence is operably linked to a promoter that drives expression of a coding sequence in a plant cell,
and wherein the transgenic plant has increased resistance to at least one nematode relative to a plant of the same species that does not comprise the nucleotide sequence.
2. The plant of claim 1 , wherein the promoter is a constitutive promoter.
3. The plant of claim 1 , wherein the plant is selected from the group consisting of rapeseed, soy, corn, sunflower, cotton, cocoa, safflower, oil palm, coconut palm, flax, castor and peanut, tomato, carrot, lettuce, bean, asparagus, cauliflower, pepper, beetroot, cabbage, eggplant, endive, leek, long cucumber, melon, pea, radish, rootstock, short cucumber, squash, watermelon, white onion, witloof, yellow onion, broccoli, brussel sprout, bunching onion, celery, mache, cucumber, fennel, gourd, pumpkin, sweet corn, and zucchini.
4. A method of producing a plant with increased nematode resistance, said method comprising:
a) introducing into the plant or a cell thereof at least one plant transformation vector comprising a nucleotide sequence that encodes an NMR polypeptide having at least 90% sequence identity to the amino acid sequence set forth as SEQ ID NO: 62, and,
b) growing the transformed plant or cell to produce a transgenic plant, wherein said transgenic plant exhibits increased resistance to at least one nematode relative to a plant of the same species that does not comprise the nucleotide sequence.
5. A transformed plant having increased nematode resistance obtained by the method of claim 4 .
6. A transformed plant part obtained from the plant according to claim 5 .
7. Transformed seed of the plant of claim 5 .