Composition and method for treatment of tumors
View Patent ↗The present invention relates to a composition which is useful in the treatment of a tumor, a method for making such a composition, and a method for using such a composition. The invention relates also to a method for assaying for inhibitors of the activity of Core 1 protein and/or other proteins of the respiratory complex III of mitochondria.
1. A composition comprising:
(A) an inhibitor of a Core 1 protein having the amino acid sequence of SEQ ID NO: 2;
(B) a member of the tumor necrosis factor superfamily; and
(C) a carrier;
wherein the inhibitor of Core 1 is a nucleic acid molecule.
2. The composition according to claim 1 , wherein the inhibitor is an antisense nucleic acid which inhibits the expression of the gene encoding Core 1.
3. The composition according to claim 1 , wherein the inhibitor is a nucleic acid which encodes an antisense nucleic acid which inhibits the expression of the gene encoding Core 1.
4. The composition according to claim 3 , wherein the nucleic acid includes a promoter region.
5. The composition according to claim 3 , wherein the nucleic acid is contained in a vector.
6. A composition according to claim 1 , wherein the inhibitor is an siRNA which inhibits the expression of the gene encoding the Core 1 protein.
7. A composition according to claim 1 , wherein the inhibitor is a nucleic acid which encodes an antibody that is capable of binding to and lowering the activity of the Core 1 protein.
8. A composition according to claim 7 , wherein the nucleic acid includes a promoter.
9. A composition according to claim 7 , wherein the nucleic acid is contained in a vector.
10. A composition according to claim 1 , wherein the inhibitor is a ribozyme that is capable of lowering the expression of the Core 1 protein.
11. A composition according to claim 1 , wherein the inhibitor is a nucleic acid which encodes a ribozyme that is capable of lowering the expression of the Core 1 protein.
12. A composition according to claim 11 , wherein the nucleic acid includes a promoter.
13. A composition according to claim 11 , wherein the nucleic acid is contained in a vector.
14. The composition according to claim 1 , wherein the member of the tumor necrosis factor superfamily is TRAIL.
15. The composition according to claim 1 , wherein the member of the tumor necrosis factor superfamily is Fas ligand.
16. A method for treating a tumor in a patient comprising the step of administering to said patient the composition of claim 1 ;
wherein the inhibitor of Core 1 inhibits the expression of the gene encoding Core 1 or the activity of Core 1 protein, thereby treating the tumor in the patient.
17. A method according to claim 16 , wherein the member of the tumor necrosis factor superfamily is TRAIL.
18. A method according to claim 16 , wherein the member of the tumor necrosis factor superfamily is Fas ligand.
19. A method for making the composition of claim 1 , comprising mixing the inhibitor of a Core 1 protein having the amino acid sequence of SEQ ID NO: 2, the carrier, and the member of the tumor necrosis factor superfamily; wherein the inhibitor of Core 1 is a nucleic acid molecule.