Use of bacterial amylases in feed for bovine animals
The invention relates to the use of at least one bacterial amylase in feed for ruminant animals of the subfamily Bovinae in particular for improving milk yield, apparent digestibility of the diet fed, feedstuff dry matter disappearance, weight gain, and/or Feed Conversion Ratio (FCR). Examples of bovine animals are dairy cows and beef cattle. The invention also relates to the use of such amylases in feed and feed additives such as premix, concentrates and total mixed ration (TMR). The amylase may be used in combination with cellulase for improving milk yield and/or back fat thickness. Preferred amylases are derived from Bacillus halmapalus, licheniformis , and stearothermophilus and are preferably homologous to Bacillus stearothermophilus amylase.
1. A method for increasing milk yield or improving organic matter digestibility in a dairy cow or beef cattle, comprising feeding the dairy cow or beef cattle with a feed which comprises an alpha-amylase, wherein
the alpha-amylase is selected from the group consisting of the sequence of amino acids 1-481 of SEQ ID NO: 2, the sequence of amino acids 1-484 of SEQ ID NO: 2, the sequence of amino acids 1-486 of SEQ ID NO: 2, and the sequence of amino acids 1-513 of SEQ ID NO: 2; and
the alpha-amylase is added in an amount to increase milk yield or to improve organic matter digestibility in a dairy cow or beef cattle, wherein the amount is in the range of 3-20 mg enzyme protein/kg diet dry matter.
2. The method of claim 1 , wherein the alpha-amylase has the sequence of 1-481 of SEQ ID NO: 2.
3. The method of claim 1 , wherein the alpha-amylase has the sequence of 1-484 of SEQ ID NO: 2.
4. The method of claim 1 , wherein the alpha-amylase has the sequence of 1-486 of SEQ ID NO: 2.
5. The method of claim 1 , wherein the alpha-amylase has the sequence of 1-513 of SEQ ID NO: 2.
6. The method of claim 1 , wherein the feed further comprises a cellulase.
7. The method of claim 1 , wherein the feed further comprises a vitamin and/or mineral.
8. The method of claim 1 , wherein the feed further comprises at least one additional ingredient selected from the group consisting of hay, forage, and roughage.
9. The method of claim 1 , wherein the feed further comprises maize and/or sorghum.
10. The method of claim 1 , wherein the alpha-amylase is added in an amount in the range of 4-15 mg enzyme protein/kg diet dry matter.