Device for controlling light radiation
View Patent ↗Device for controlling light radiation, which is excited in a specimen and/or which is backscattered and/or reflected and which contains one or more wavelengths, at a plurality of light outlets, wherein a separation of the light radiation into differently polarized components is carried out; and the components of the excitation radiation and/or detection radiation are affected in their polarization by means of a preferably birefringent, preferably acousto-optic or electro-optic medium, which changes the ordinary and extraordinary refractive index.
1. A microscope having a light source for illuminating a specimen, means for detecting light coming from the specimen, and a device for controlling light radiation coming from at least one of the light source and the specimen, the device comprising:
a plurality of light outlets;
first polarizing means for spatially separating the light radiation into components of different polarization;
a collinear acousto-optic tunable filter downstream of the first polarizing means for affecting the polarization of the components in a wavelength-dependent manner, wherein the light radiation incident on the acousto-optic tunable filter is in a condition free of dispersion, and wherein the acousto-optic tunable filter passes the light radiation in a condition free of dispersion; and
second polarizing means downstream of the acousto-optic tunable filter for combining the components of different polarization while spatially separating affected and unaffected wavelengths of the components to different light outlets.
2. The microscope as claimed in claim 1 , wherein the first polarizing means are arranged for spatially separating the light radiation coming from the light source into illumination components of different polarization;
wherein the second polarizing means are arranged for combining the illumination components while spatially separating affected and unaffected wavelengths of the illumination components to the different light outlets and for spatially separating the light radiation coming from the specimen into detection components of different polarization; and
wherein the first polarizing means are arranged for combining the detection components while spatially separating affected and unaffected wavelengths of the detection components to different light outlets.
3. The microscope as claimed in claim 1 , wherein the acousto-optic tunable filter is constructed so that it does not spectrally split the light radiation passing therethrough.
4. The microscope as claimed in claim 1 , wherein the light source is a laser and wherein the microscope further comprises means for laser-scanning the specimen.
5. The device, as claimed in claim 1 , further comprising means for descanned detection of radiation light coming from the specimen.
6. The device, as claimed in claim 1 , further comprising means for partial descanned detection in one direction of radiation light coming from the specimen.
7. The device, as claimed in claim 1 , further comprising means for non-descanned detection of radiation light coming from the specimen.
8. The device, as claimed in claim 1 , further comprising a line detector for spectrally resolved measurement.
9. The device, as claimed in claim 1 , wherein the first polarizing means and the second polarizing means are provided by a single polarization-splitting element.
10. A microscope having a light source for illuminating a specimen, means for detecting light coming from the specimen, and a device for controlling light radiation coming from at least one of the light source and the specimen, the device comprising:
a plurality of light outlets;
first polarizing means for spatially separating the light radiation into components of different polarization;
a collinear acousto-optic tunable filter downstream of the first polarizing means for affecting the polarization of the components in a wavelength-dependent manner, and
second polarizing means downstream of the acousto-optic tunable filter for combining the components of different polarization while spatially separating affected and unaffected wavelengths of the components to different light outlets,
wherein between the first polarizing means and the second polarizing means, the device is free from dispersion means.