Method for forming polymerized microfluidic devices
View Patent ↗Methods for making a micofluidic device according to embodiments of the present invention include defining a cavity. Polymer precursor solution is positioned in the cavity, and exposed to light to begin the polymerization process and define a microchannel. In some embodiments, after the polymerization process is partially complete, a solvent rinse is performed, or fresh polymer precursor introduced into the microchannel. This may promote removal of unpolymerized material from the microchannel and enable smaller feature sizes. The polymer precursor solution may contain an iniferter. Polymerized features therefore may be capped with the iniferter, which is photoactive. The iniferter may aid later binding of a polyacrylamide gel to the microchannel surface.
1. A manifold structure comprising:
a first substrate having a first surface, the first surface having an iniferter species attached to the surface;
a second substrate having a first surface and a second surface, the second substrate having a plurality of access ports, each extending from the first surface to the second surface, the first surface having an iniferter species attached to the surface;
a spacer between the first and second substrates such that the first and second substrates and the spacer define a cavity, the cavity partially defined by the first surface of the first substrate and the first surface of the second substrate; and
polymerized polymer precursor disposed in the cavity, wherein the polymerized polymer precursor includes a monomeric species, a photoinitiator, and an iniferter species, wherein the polymerized polymer precursor defines at least one fluidic feature in fluid communication with at least one of the plurality of access ports, and wherein at least a portion of the polymerized polymer precursor defines a polymer surface having at least some of the iniferter species on the polymer surface; and
wherein a first one of the plurality of access ports is configured to supply an unpolymerized polymer precursor solution to the cavity, at least one of the first and second substrates is sufficiently transparent to allow illumination of the cavity through the transparent substrate to polymerize a region of the unpolymerized polymer precursor in the cavity, and wherein a second one of the plurality of access ports is configured to remove partially polymerized polymer precursor solution from the cavity.
2. The manifold structure according to claim 1 further comprising:
a photomask defining the region of the cavity for polymerization, the photomask placed between the transparent substrate and a light source.
3. The manifold structure according to claim 2 further comprising:
a monomer injection block having a plurality access ports configured to couple to the access ports of the second substrate; and
a fluid manifold coupled to the monomer injection block for fluidic coupling to the cavity.
4. The manifold structure according to claim 3 further comprising a compression frame and fasteners applying compressive force to the fluid manifold, the monomer injection block, the second substrate, the spacers, the first substrate, and the photomask.
5. The manifold structure according to claim 4 further comprising a gasket between the monomer injection block and the second substrate.
6. The manifold structure according to claim 1 wherein the spacer has a thickness of less than 200 μm.
7. The manifold structure according to claim 1 wherein the iniferter comprises a TED.
8. The manifold structure according to claim 1 wherein the at least one of the first and second access ports is further configured to supply an aqueous acrylamide solution to the cavity.
9. The manifold structure according to claim 1 further comprising:
a polyacrylamide gel disposed in at least a portion of the fluidic feature defined by the polymerized polymer precursor.
10. The manifold structure according to claim 9 wherein the polyacrylamide gel is in contact with the polymer surface having at least some of the iniferter species on the polymer surface.
11. The manifold structure according to claim 1 further comprising a pump configured to flow partially polymerized polymer precursor solution through the fluidic feature during polymerization of the polymer precursor.