Proteoliposome, production method thereof, and biochip
View Patent ↗A proteoliposome, which is obtained by removing a surfactant from a mixed solution including lipid, membrane proteins and the surfactant, wherein the content of the surfactant in the mixed solution is equal to or more than 1.5 times of the sum of a maximum amount of the surfactant associating with the lipid and a maximum amount of the surfactant associating with the membrane protein. A biochip wherein the above-described proteoliposome is spread on a substrate. A method for producing a proteoliposome by removing a surfactant from a mixed solution including lipid, membrane proteins and the surfactant, wherein the content of the surfactant in the mixed solution is made equal to or more than the sum of a maximum amount of the surfactant associating with the lipid and a maximum amount of the surfactant associating with the membrane protein.
1. A method for producing a proteoliposome by mixing lipid, membrane proteins and a surfactant to prepare a mixed solution, then removing the surfactant from the mixed solution,
wherein the content of the surfactant in the mixed solution is made 2.0 times or more than the sum of a maximum amount of the surfactant associating with the lipid plus a maximum amount of the surfactant associating with the membrane protein,
wherein a content of lipid in the mixed solution is 2.2 to 3.0 mM based on the total amount of the mixed solution,
wherein a content of the membrane protein in the mixed solution is 83 to 145 nM based on the total amount of the mixed solution,
wherein the lipid is at least one selected from the group consisting of phosphatidylcholine and phosphatidylserine,
wherein the membrane protein is an ionotropic receptor, and
wherein the surfactant is a nonionic surfactant.
2. The method of claim 1 , further comprising spreading the proteoliposome on a substrate to produce a biochip.
3. The method according to claim 2 , wherein the substrate includes at least one kind selected from the group consisting of mica, SiO 2 , SiN, Au and Pt.
4. The method according to claim 1 , wherein the membrane protein is an AMPA receptor.
5. The method according to claim 1 , wherein the surfactant is a n-octyl-D-glucopyranoside.