Plants having tomato torrado virus resistance
View Patent ↗The present invention relates to a tomato plant having within its genome at least one allele of a gene that confers resistance to Tomato torrado virus (ToTV), said virus having been deposited with the Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH on 24 Nov. 2004 under depositors reference number ToTV-E01 (DSM 16999).
1. A hybrid tomato plant of the species Solanum lycopersicum comprising commercially desirable characteristics, obtained by crossing a donor plant having within its genome a haplotype that confers resistance to tomato torrado virus (ToTV), as found in tomato breeding line T06PF1, a strain of said virus having been deposited with the Deutsche Sammlung von Mikroorganismen and Zellkulturen GmbH on 24 Nov. 2004 under depositor's reference number ToTV-E01 (DSM 16999), and a deposit of seed of said tomato line having been deposited with the American type Culture Collection (ATCC) on 6 Mar. 2014 under depositor's reference number 11 EFT 41822 (PTA-121067), wherein said haplotype is located in a genomic region of chromosome 4 between markers CT264 and TG163, linked to one or more markers selected from the group consisting of P14/M49-F-282, P11/M54-F-233/235, P11/M35-F-216, P21/M61-F-583, P25/M51-F-131, P015/M49-F-330, P13/M38-F-311/313 and C2_At5g25900, with a recipient plant comprising commercially desirable characteristics, wherein said obtained plant is produced by a method comprising screening for the presence of at least one molecular marker linked to said ToTV resistance haplotype, and wherein said haplotype is present in homozygous form.
2. The plant according to claim 1 , wherein said resistance is expressed as a resistance to establishment of an infection.
3. A method of identifying the plant of claim 1 , said method comprising performing a resistance bioassay using infection with said ToTV on offspring of a parental plant having within its genome a haplotype that confers resistance to tomato torrado virus (ToTV), as found in tomato breeding line T06PF1.
4. The plant according to claim 1 , wherein the at least one molecular marker is selected from the group consisting of one or more of P14/M49-F-282, P11/M35-F-216, P21/M61-F-583, P25/M51-F-131, P15/M49-F-330, P13/M38-F-311/313 and C2_At5g25900.
5. A plant part of the plant of claim 1 , comprising said haplotype.
6. The plant part according to claim 5 , wherein said part is a fruit or a seed.
7. A tomato plant of the species Solanum lycopersicum obtained by crossing a donor plant having within its genome a haplotype as found in tomato breeding line T06PF1, a deposit of seed of said tomato line having been deposited with the American type Culture Collection (ATCC) on 6 Mar. 2014 under depositor's reference number 11 EFT 41822 (PTA-121067), in homozygous form that confers resistance to tomato torrado virus (ToTV) with a recipient plant comprising commercially desirable characteristics, wherein said haplotype is located in a genomic region of chromosome 4 between markers CT264 and TG163 linked to one or more markers selected from the group consisting of P14/M49-F-282, P11/M54-F-233/235, P11/M35-F-216, P21/M61-F-583, P25/M51-F-131, P15/M49-F-330, P13/M38-F-311/313 and C2_At5g25900, wherein said obtained plant is obtained by
a) providing an inoculum of said ToTV;
b) exposing a tomato plant to said inoculum;
c) allowing for a sufficient incubation time; and
d) selecting said tomato plant having within its genome the haplotype that confers resistance to ToTV if no infection establishes in said plant upon lapse of said incubation time.
8. A plant part of the plant of claim 1 , comprising said haplotype.
9. The plant part according to claim 8 , wherein said part is a fruit or a seed.
10. The plant according to claim 1 , wherein the one or more markers are selected from the group consisting of P14/M49-F-282, P11/M35-F-216 and P13/M38-F-311/313.
11. The plant according to claim 1 , wherein the one or more markers are selected from the group consisting of P11/M35-F-216, P13/M38-F-311/313 and C2_At5g25900.
12. The plant according to claim 1 , wherein said haplotype is located in a genomic region positioned between AFLP markers P14/M49-F-282 and P11/M35-F-216 on chromosome 4.
13. The plant according to claim 1 , wherein said haplotype is located in a genomic region positioned AFLP marker P13/M38-F-311/313 and COSII/CAPS marker C2 _At5g25900 on chromosome 4.
14. The plant according to claim 1 , wherein said at least one molecular marker is selected from the group consisting of one or more of P14/M49-F-282, P11/M35-F-216, P13/M38-F-311/313 and C2_At5g25900.
15. The plant according to claim 1 , wherein said at least one molecular marker is selected from the group consisting of one or more of P11/M35-F-216, P13/M38-F-311/313 and C2_At5g25900.
16. The plant of claim 1 , wherein the haplotype is linked within 10 cM of markers P13/M38-F-311/313 and C2_At5g25900.
17. The plant of claim 1 , wherein the haplotype is linked within 3.6 cM of markers P13/M38-F-311/313 and C2_At5g25900.