Crystalline form II of tigecycline and processes for preparation thereof
The present invention provides processes for the preparation of crystalline forms of Tigecycline.
1. A crystalline tigecycline form II, characterized by data selected from the group consisting of: a powder XRD pattern with peaks at 9.5, 9.8, 18.1, 20.2 and 21.6±0.2 degrees two-theta; a powder XRD spectrum as depicted in FIG. 1 ; a solid-state 13 C NMR spectrum with signals at 197.9, 149.1, 143.9, 115.2, and 106.0±0.2 ppm; a solid-state 13 C NMR spectrum having chemical shift differences between the signal exhibiting the lowest chemical shift and another in the chemical shift range of 90 to 210 ppm of 98.1, 49.3, 44.1, 15.4, and 6.2±0.1 ppm wherein the signal exhibiting the lowest chemical shift in the chemical shift range of 90 to 210 ppm is at 99.8±1 ppm; a solid-state 13 C NMR spectrum depicted in FIG. 2 ; and a solid-state 13 C NMR spectrum depicted in FIG. 3 .
2. The crystalline tigecycline of claim 1 , further comprising water at an amount of more than 1.2% by weight as measured by Karl Fisher (KF).
3. The crystalline tigecycline of claim 1 , having a water content of about 1.2% to about 3.0% by weight as measured by KF.
4. The crystalline tigecycline of claim 3 , having a water content of about 1.3% to about 2.0% by weight as measured by KF.
5. A process of preparing crystalline tigecycline form II of claim 1 comprising: preparing crystalline tigecycline form I, characterized by a powder XRD pattern with peaks at 4.2, 9.1, 11.4, 14.0, and 15.7±0.2 degrees two-theta, comprising:
a) providing a solution of tigecycline in dichloromethane (DCM);
b) performing a solvent exchange of DCM with a C3-C6 ester to obtain a suspension; and
c) cooling the suspension to obtain crystalline tigecycline form I;
and slurrying said crystalline tigecycline form I with a solvent selected from the group consisting of acetonitrile and a C3-C6 ketone to obtain crystalline tigecycline form II.
6. The process of claim 5 , further comprising drying the crystalline tigecycline form II under a vacuum at 50° C. to about 80° C. for a period of about 3-120 hours.
7. The process of claim 6 , wherein the period is about 3-9 hours.
8. The process of claim 5 , wherein slurrying is performed at about room temperature for a period of about 8 to about 16 hours.
9. The process of claim 8 , wherein slurrying is performed at about 20-25° C.
10. The process of claim 5 , wherein slurrying is performed with acetone or acetonitrile.
11. A process of preparing crystalline tigecycline form II of claim 1 comprising: a) providing a solution of tigecycline in dichloromethane (DCM); b) performing a solvent exchange of DCM with a solvent selected from the group consisting of: acetonitrile and a C 3 -C 6 ketone to obtain a suspension; and c) cooling the suspension to obtain crystalline tigecycline form II.
12. The process of claim 11 further comprising drying the obtained tigecycline form II under a vacuum at about 50° C. to about 80° C. for a period of about 3-120 hours.
13. The process of claim 12 , wherein the period is about 3-9 hours.
14. The process of claim 12 , wherein the period is about 12 hours.
15. The process of claim 11 , wherein the solvent in step b) is acetonitrile or acetone.
16. The process of claim 11 , wherein cooling is performed for about 15-90 minutes to a temperature of about −5° C. to about 10° C.
17. The process of claim 16 wherein the cooling is performed to a temperature of about 0° C. to about 5° C.
18. A process of preparing the crystalline tigecycline of claim 2 comprising: exposing tigecycline form II at room temperature to a humid environment for a sufficient duration of time.
19. The process of claim 18 , wherein the tigecycline form II is exposed to a humid environment at a temperature of about 20° C. to about 25° C. for a period of about 4 days to about 14 days.
20. The process of claim 18 , wherein the humid environment has a relative humidity about 40%-80%.
21. The process of claim 20 wherein the humid environment has a relative humidity of about 80%.
22. The process of claim 18 , wherein a crystalline tigecycline having a water content of more than 1.2% by weight as measured by Karl Fisher (KF) is obtained.
23. The process of claim 18 , wherein a crystalline tigecycline having a water content of about 1.2% to about 3.0% by weight as measured by Karl Fisher (KF) is obtained.
24. The process of claim 23 , wherein a crystalline tigecycline having a water content of about 1.3% to about 2.0% by weight as measured by Karl Fisher (KF) is obtained.
25. A pharmaceutical formulation comprising crystalline Tigecycline form II of claim 1 and at least one pharmaceutically acceptable excipient.
26. A pharmaceutical composition comprising crystalline Tigecycline form II of claim 1 obtained by the process of claim 5 , 11 , or 18 , and one or more pharmaceutically acceptable excipients.
27. A process for preparing a pharmaceutical formulation comprising combining crystalline Tigecycline form II of claim 1 , with at least one pharmaceutically acceptable excipient.