SEED-PREFERRED GENE PROMOTERS FROM THE CASTOR PLANT
A nucleotide sequence comprising a nucleotide sequence having at least about 90% homology to the sequence of SEQ ID NO:9 or SEQ ID NO:10. Nucleotide sequences interest operatively linked to nucleotide sequence having at least about 90% homology to the sequence of SEQ ID NO:9 or SEQ ID NO:10 are disclosed. Vectors, methods of regulating target expression, methods of providing a cells, plants, and seeds comprising the nucleotide sequence are also disclosed.
1 . An isolated nucleotide sequence comprising a nucleotide sequence having at least about 90% homology to the sequence of SEQ ID NO:9 or SEQ ID NO:10.
2 . The isolated nucleotide sequence of claim 1 , further comprising a nucleotide sequence of interest operatively linked to the nucleotide sequence having at least about 90% homology to the sequence of SEQ ID NO:9 or SEQ ID NO:10.
3 . The isolated nucleotide sequence of claim 2 , wherein the nucleotide sequence of interest encodes a molecule selected from the group consisting of proteins, antisense oligonucleotides, and siRNAs.
4 . A plant comprising the isolated nucleotide sequence of claim 1 .
5 . The plant according to claim 4 , wherein the plant is selected from the group consisting of Arabidopsis sp., sunflower, cotton cells, rapeseed, maize, palm, tobacco, peanut, soybean, and Ricinus sp.
6 . The plant according to claim 4 , wherein the plant comprises the vector of claim 4 .
7 . A genetic descendant of the plant of claim 4 .
8 . A seed comprising the isolated nucleotide sequence of claim 1 .
9 . The seed according to claim 8 , wherein the seed is selected from the group consisting of Arabidopsis sp., sunflower, cotton cells, rapeseed, maize, palm, tobacco, peanut, soybean, and Ricinus sp. seeds.
10 . The seed according to claim 8 , wherein the plant comprises the vector of claim 4 .
11 . The seed according to claim 8 , wherein the plant comprises the cell of claim 9 .
12 . A method of promoting the expression of a molecule encoded by a nucleotide sequence of interest, the method comprising: operatively linking the isolated nucleotide sequence of claim 1 to the nucleotide sequence of interest.
13 . The method according to claim 12 , wherein the expression of the molecule encoded by the nucleotide sequence of interest is promoted in a seed preferred manner.
14 . The method of claim 12 , wherein the molecule encoded by the nucleotide sequence of interest is selected from the group consisting of a proteins, antisense oligonucleotides, and siRNAs.
15 . A method of modulating the expression of a target in a cell, the method comprising:
providing a nucleotide sequence of interest, wherein the nucleotide sequence encodes an antisense oligonucleotide or an siRNA that is capable of modulating the expression of the target in a cell;
operatively linking the isolated nucleotide sequence of claim 1 to the nucleotide sequence of interest;
providing the operatively linked nucleotide sequences to the cell; and
expressing the nucleotide sequence of interest.
16 . The method according to claim 15 , wherein the cell is selected form the group consisting of prokaryotic, eukaryotic, bacterial, agrobactrerium, yeast, plant, mammalian, and human cells.
17 . The method according to claim 15 , herein the plant cell is selected form the group consisting of Ricinus, Arabidopsis, sunflower, cotton, rapeseed, maize, palm, tobacco, peanut or soybean cells.
18 . The method according to claim 15 , wherein the target is a gene, oligonucleotide sequence, and/or a protein.
19 . The method according to claim 15 , wherein the target is selected from proteins involved in fatty acid synthesis, degradation, storage, and/or regulation
20 . The method according to claim 15 , wherein the target is selected from the group consisting of ACCase, FAS, KAS I, KAS II, KAS III, Fad2, and Fad3.